10108014001

Papain

from Carica papaya

• 동의어(들): cystein protease, papain
• 효소 위원회 번호: 3.4.22.2 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: papaya
• Quality Level: 100
• 형태: suspension
• 특이 활성도: 30 U/mg; ~30 units/mg protein (At 25 °C with BAEE as the substrate.)
• 분자량: 23 kDa
• 포장: pkg of 10 mL (100 mg)
• 제조업체/상표: Roche
• 농도: 10-100 mg/mL
• 최적 pH: 6.0-7.0
• 배송 상태: wet ice

설명

일반 설명

Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.

애플리케이션

Papain has been used in the digestion of various brain cells.

Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.

생화학적/생리학적 작용

Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.

특징 및 장점

Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals

물리적 형태

Suspension, crystalline, nonsterile

제조 메모

Working concentration: 0.05 to 0.5 mg/ml

저장 및 안정성

Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash