10109118001

Reverse Transcriptase AMV

solution, >50 units/μg protein, suitable for RT-qPCR, suitable for RT-PCR

• 동의어(들): amv reverse transcriptase
• 효소 위원회 번호: 2.7.7.49 (BRENDA, IUBMB)
• UNSPSC 코드: 41105600

속성

• 형태: solution
• Quality Level: 100
• 특이 활성도: >50 units/μg protein
• 특징: dNTPs included: no; hotstart: no
• 포장: pkg of 1,000 U
• 제조업체/상표: Roche
• 저장 조건: avoid repeated freeze/thaw cycles
• 파라미터: 42 °C optimum reaction temp.
• 기술: RT-PCR: suitable; RT-qPCR: suitable
• 입력: purified RNA
• 검출 방법: probe-based

설명

일반 설명

Reverse Transcriptase, AMV is a gene product of the RNA genome of avian myeloblastosis virus. The enzymatically active forms of the purified enzyme are α,ββ and αβ. The molecular weight of the α-subunit is 68 kDa, that of the β-subunit 92 kDa. The mature αβ form, the most active form of Reverse Transcriptase, AMV, includes a RNA-directed DNA polymerase, a DNA-dependent DNA polymerase, a RNase H, and an unwinding activity. Reverse Transcriptase, AMV is used for cDNA synthesis, for synthesis of first strand cDNA for use in subsequent amplification reactions and dideoxy DNA sequencing.
The enzyme can also be used for RNA sequencing, 3′ end labeling of DNA fragments, and the generation of ss probes for genomic footprints.

Reverse Transcriptase AMV requires a primer and Mg²⁺ or Mn²⁺ for activity.

특이성

AMV (Avian Myeloblastosis Virus) Reverse Transcriptase is an RNA-directed DNA polymerase and a DNA-dependent DNA polymerase. The AMV reverse transcriptase requires a primer and Mg²⁺ or Mn²⁺ for activity.
Heat inactivation: 5 min, 95 °C

애플리케이션

Reverse Transcriptase AMV is suitable for:

• First- and second-strand cDNA synthesis and synthesis of first strand cDNA for use in subsequent amplification reactions (RT-PCR)
• Dideoxy DNA sequencing
• Primer extension
• RNA sequencing
• 3′-end labeling of DNA fragments
• Generation of single-stranded probes for genomic footprint experiments

특징 및 장점

• Efficiently transcribes total RNA, mRNA, viral RNA and RNA rich in secondary structures
• Procure full length cDNA fragments up to 12 kb
• Higher thermostability (up to 60°C) and specificity than M-MuLV Reverse Transcriptase

포장

1 kit containing 2 components

품질

Absence of contaminants: Tested for the absence of detectable nonspecific RNases and nonspecific DNases in incubations with various nucleic acids (analyzed by gel electrophoresis) according to the current Quality Control procedures.
Function test: Reverse Transcriptase AMV is function tested in the cDNA Synthesis Kit and RT-PCR.

단위 정의

1 unit is the enzyme activity that incorporates 1 nmol of [³H]-dTMP into acid-precipitable products in 10 minutes at +37 °C with poly(A)⁺ · d(pT)₁₅ as template primer.

Volume Activity: 20-25 U/μl. Please refer to the Certificate of Analysis for more information.

제조 메모

Storage buffer: 200 mM potassium phosphate, 2 mM dithiothreitol, 0.2% Triton X-100 (v/v), 50% glycerol (v/v), pH 7.2.

저장 및 안정성

Store at -15–-25 °C. (Storage at -70 °C is more likely to deteriorate the enzyme via repeated freezing and thawing.)

기타 정보

For life science research only. Not for use in diagnostic procedures.

키트 구성품 전용

• Reverse Transcriptase AMV 20-25 U/μl
• First-strand cDNA Synthesis Buffer 5x concentrated

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash