10236276001

DAPI

4′,6-Diamidine-2′-phenylindole dihydrochloride

• 동의어(들): 4′,6-Diamidino-2-phenylindole dihydrochloride, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
• 실험식(Hill 표기법): C₁₆H₁₅N₅ · 2HCl
• CAS Number: 28718-90-3
• Molecular Weight: 350.25
• Beilstein: 4894417
• MDL number: MFCD00012681
• UNSPSC 코드: 41116100
• PubChem Substance ID: 329748990

속성

• Quality Level: 100
• 분석: >90%
• 형태: powder
• 포장: pkg of 10 mg
• 제조업체/상표: Roche
• λmax: 340 nm in aq. suspension
• 형광: λ_ex 340 nm; λ_em 488 nm (nur DAPI); λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex)
• 저장 온도: room temp
• SMILES string: Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
• InChI: 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
• InChI key: FPNZBYLXNYPRLR-UHFFFAOYSA-N

설명

애플리케이션

DAPI is a fluorescent dye that binds selectively to double-stranded DNA and forms strongly fluorescent DNA-DAPI complexes with high specificity. It is commonly used to detect mycoplasma in cell culture via fluorescence microscopy.

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

생화학적/생리학적 작용

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

품질

Purity: >90% (from N)

원리

The fluorescent dye DAPI binds selectively to DNA and forms strongly fluorescent DNA-DAPI complexes with high specificity. DAPI, once added to tissue culture cells, is rapidly taken up into cellular DNA, yielding highly fluorescent nuclei and no detectable cytoplasmic fluorescence. When the cells are contaminated with Mycoplasmas, characteristic discrete fluorescent foci are readily detected over the cytoplasm and sometimes in intercellular spaces.

제조 메모

Working solution: Solubility: 25 mg/ml in water


Preparation of stock solution

Dissolve in double-dist. water to a final concentration of 1 to 5 mg/ml.
Note: Do not use any buffers.


Preparation of working solution

Dilute the stock solution with methanol to a final concentration of 1 μg/ml. The working solution is stable at 2 to 8 °C for about 6 months.
Storage conditions (working solution): Stock solution (1 to 5 mg/ml) at -15 to -25 °C for 12 months.
Working solution (1μg/ml) at 2 to 8 °C for about 6 months.

재구성

In 2 to 10 ml double-dist. water; 1 to 5 mg/ml final concentration.
Note: Prepare aliquots and store at -15 to -25 °C.

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable