추천 제품
양식
solution
Quality Level
포장
pkg of 50 μg (in 200 μl)
제조업체/상표
Roche
농도
250 μg/mL
색상
colorless
solubility
water: miscible
저장 온도
−20°C
일반 설명
DNA Molecular Weight Marker VI is a fragment mixture prepared by cleavage of pBR328 DNA with restriction endonucleases Bgl I and pBR328 DNA digested with Hinf I. It has a size range of 0.15 to 2.1kbp.
The product is also available as DNA Molecular Weight Marker VI, Digoxigenin-labeled.
The product is also available as DNA Molecular Weight Marker VI, Digoxigenin-labeled.
특이성
Specificity: Product has 5′-phosphorylated ends (restriction fragments).
Restriction site: Claevage sites and corresponding fragment lengths of pBR328:
- Bgl I cut sites at: 935, 1169, 2399, 4575, 4809 => creating following fragment sizes, respectively: 234 bp, 1230 bp, 2176 bp, 234 bp, 1033 bp.
- Hinf I cut sites at: 632, 852, 1006, 1304, 1757, 2274, 4040, 4434, 4732, 4886. =>
creating following fragment sizes, respectively: 220 bp, 154 bp, 298 bp, 453 bp, 517 bp,
1766 bp, 394 bp, 298 bp, 154 bp, 653 bp
Restriction site: Claevage sites and corresponding fragment lengths of pBR328:
- Bgl I cut sites at: 935, 1169, 2399, 4575, 4809 => creating following fragment sizes, respectively: 234 bp, 1230 bp, 2176 bp, 234 bp, 1033 bp.
- Hinf I cut sites at: 632, 852, 1006, 1304, 1757, 2274, 4040, 4434, 4732, 4886. =>
creating following fragment sizes, respectively: 220 bp, 154 bp, 298 bp, 453 bp, 517 bp,
1766 bp, 394 bp, 298 bp, 154 bp, 653 bp
애플리케이션
DNA Molecular Weight Marker VI is used for the size determination of DNA in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:
- Restriction digests
- PCR
- RT-PCR
서열
As determined by computer analysis of the pBR328 sequence, the mixture contains 15 DNA fragments with the following base pair lengths (1 base pair = 660 daltons) occuring once: 220, 394, 453, 517, 653, 1033, 1230, 1766, 2176 and fragments 154,234, and 298 occuring twice as a result of the digestion of pBR328 with both Bgl I and Hinf I.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
단위 정의
50 μg = 1A260 unit
물리적 형태
Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).
제조 메모
Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.
Once thawed we recommend further storage at 2 to 8 °C.
기타 정보
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
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