B9431

Biotin-4-Fluorescein

≥95% purity (HPLC), powder

• 동의어(들): 5-(6)-[2-(biotinoyl)aminoethylaminocarbonyl] fluorescein, B4F, Biotin (5-fluorescein) conjugate
• 실험식(Hill 표기법): C₃₃H₃₂N₄O₈S
• Molecular Weight: 644.69
• MDL number: MFCD09264223
• UNSPSC 코드: 12171500
• eCl@ss: 34058011
• PubChem Substance ID: 329773319
• NACRES: NA.47

속성

• 제품명: Biotin-4-Fluorescein, ≥95.0% (HPLC)
• Quality Level: 100; 200
• 분석: ≥95.0% (HPLC)
• 양식: powder
• 분자량: 644.71
• 기술: titration: suitable
• 색상: light orange to very dark orange; light yellow to very dark yellow
• ε (흡광계수): ≥70 at 491-497 nm in phosphate buffer at 0.05 M
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: room temp
• SMILES string: OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4ccc(O)cc24)C(=O)NCCNC(=O)CCCC[C@@H]5SC[C@@H]6NC(=O)N[C@H]56
• InChI: 1S/C33H32N4O8S/c38-18-7-10-25-23(14-18)29(21-9-6-19(39)15-26(21)45-25)20-8-5-17(13-22(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30
• InChI key: UROXVNHIYQXTRA-LFERIPGTSA-N

설명

애플리케이션

Biotin-4-Fluorescein has been used for the labeling of avidin to study the changes in protein binding capability after carbon negative-ion implantation into avidin. It has been used to study the binding affinity of streptavidin towards biotin.

Useful for quantitating biotin binding sites. This reagent binds to avidin more quickly than conventional fluorescein biotin.

생화학적/생리학적 작용

Biotin-fluorescein is mainly used for the assay of biotin-interaction sites in avidin and streptavidin. Binding of biotin-4-fluorescein results in strong quenching, which is used for measuring the concentration of (strept)avidin.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)