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Merck
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Key Documents

01632

Sigma-Aldrich

Atto 520-Biotin

BioReagent, suitable for fluorescence, ≥90% (HPLC)

동의어(들):

Biotin-Atto 520

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About This Item

실험식(Hill 표기법):
C37H53ClN6O8S
Molecular Weight:
777.37
MDL number:
UNSPSC 코드:
12352108
eCl@ss:
34058011
PubChem Substance ID:
NACRES:
NA.32

제품 라인

BioReagent

Quality Level

분석

≥90% (HPLC)

형태

powder

제조업체/상표

ATTO-TEC GmbH

solubility

DMF: soluble
ethanol: soluble
methanol: soluble

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV 흡수

λ: 522-528 nm Amax

적합성

suitable for fluorescence

저장 온도

2-8°C

SMILES string

[O-]Cl(=O)(=O)=O.CCNc1cc2OC3=CC(=[NH+]\CC)\C(C)=CC3=C(CCC(=O)NCCCCCNC(=O)CCCCC4SCC5NC(=O)NC45)c2cc1C

InChI

1S/C37H52N6O4S.ClHO4/c1-5-38-28-20-31-26(18-23(28)3)25(27-19-24(4)29(39-6-2)21-32(27)47-31)14-15-35(45)41-17-11-7-10-16-40-34(44)13-9-8-12-33-36-30(22-48-33)42-37(46)43-36;2-1(3,4)5/h18-21,30,33,36,38H,5-17,22H2,1-4H3,(H,40,44)(H,41,45)(H2,42,43,46);(H,2,3,4,5)/b39-29-;

InChI key

OCYRRKMPTKPPHX-QITHAKLPSA-N

애플리케이션

Atto 520 is a fluorescent label with high molecular absorption (110.000) and quantum yield (0.90) as well as sufficient stoke′s shift (excitation maximum 520 nm, emission maximum 542 nm). It does nearly not show cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Can be utilized in applications such as ELISA or immuno-histochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates. Atto-520 fluorescent-labeled biotin can be loaded onto magnetic beads coated with streptavidin through specific ligand–receptor interactions.

법적 정보

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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문서

Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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