05066

Agarose

High EEO, for molecular biology

• 동의어(들): 3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE
• CAS Number: 9012-36-6
• EC Number: 232-731-8
• MDL number: MFCD00081294
• UNSPSC 코드: 41105317
• PubChem Substance ID: 24845663
• NACRES: NA.25

속성

• Grade: for molecular biology
• 형태: powder
• 불순물: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• 무기 잔류물: ≤1%
• 손실: ≤10% loss on drying
• EEO: 0.23-0.27
• 전이 온도: gel point 34-37 °C (1.5% solution)
• 겔 강도: ≥1500 g/cm²
• 음이온 미량물: chloride (Cl^-): ≤0.3%; sulfate (SO₄^2-): ≤0.6%
• 양이온 미량물: Ca: ≤0.05%; Cd: ≤0.001%; Co: ≤0.001%; Cr: ≤0.001%; Cu: ≤0.001%; Fe: ≤0.001%; K: ≤0.05%; Mg: ≤0.001%; Mn: ≤0.001%; Na: ≤0.5%; Ni: ≤0.001%; Pb: ≤0.001%; Zn: ≤0.001%
• InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
• InChI key: MJQHZNBUODTQTK-WKGBVCLCSA-N

설명

애플리케이션

Agarose is used for preparative and analytical separation of nucleic acids. It can be used for single cell gel electrophoresis assay. It can also be used for southern blotting. It can also be used for assessing transepithelial ionic fluxes from cultured neonatal rat semicircular canal epithelium.

생화학적/생리학적 작용

Agarose is a polysaccharide used for resolving DNA and RNA fragments from 500 - 20,000 bp. It provides strong gel structure which assists in better handling and less breakage. It can also reexpress the differentiated collagen phenotype from dedifferentiated chondrocytes during agarose gel culture.

포장

Packaging
50, 500 g in poly bottle

분석 메모

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)