05500

Alcian Blue 8GX

for microscopy (Bact., Bot., Hist.)

• 동의어(들): Alcian Blue, Ingrain Blue 1
• 실험식(Hill 표기법): C₅₆H₆₈Cl₄CuN₁₆S₄
• CAS Number: 33864-99-2
• Molecular Weight: 1298.86
• 색상 지수 번호: 74240
• EC Number: 251-705-7
• MDL number: MFCD00010720
• UNSPSC 코드: 12171500
• PubChem Substance ID: 329748076
• NACRES: NA.47

속성

• Grade: for microscopy (Bact., Bot., Hist.)
• Quality Level: 100
• 형태: powder
• solubility: methanol: water (1:1): 0.01 g/10 mL, blue to very deep blue
• εmax: ≥60 at 605-625 nm in water
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: room temp
• SMILES string: [Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C
• InChI: 1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4
• InChI key: CKLBXIYTBHXJEH-UHFFFAOYSA-J

설명

일반 설명

Alcian Blue 8GX (Cl 74240), also known as Alcian Blue 8GN or Ingrain Blue 1, is a cationic, copper phthalocyanine dye. Four cationic guanidinium groups are attached to the ring system via thioether links, which form an insoluble pigment when broken by hydroxide ions or nucleophiles. The dye is basic, tetravalent, and blue in color due to its copper content. It forms salt linkages with the acid groups of acid mucopolysaccharides.

애플리케이션

• Alcian Blue 8GX is generally used for the demonstration of mucopolysaccharides and as a general microscopic stain.
• It is a heteroglycan stain for neutral, sulfated, and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices.
• It has been used to discover novel insights into the short-term impact of food-relevant synthetic amorphous silica on the intestinal epithelium.
• It is suitable for amyloid staining, cystine following oxidation, and staining mast cells in polychromatic alcian blue-safranine methods.
• It is applicable as a microscopic stain for the detection of bacterial capsules, and mucin staining of cytological fine-needle aspirates.

생화학적/생리학적 작용

Alcian Blue 8GX forms insoluble complexes with acidic glycosaminoglycans, thereby helping in the quantitative determination of glycosaminoglycans.

특징 및 장점

• Desired staining patterns can be obtained by manipulating pH and salt concentrations.

원리

Alcian blue staining can be combined with the Periodic acid-Schiff (PAS) reaction. Acid mucosubstances in tissues are stained selectively and counterstained most commonly with nuclear fast red. This can be combined with the PAS reaction to additionally visualize non-substituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glycol- and phospholipids, using an Acetic acid-Alcian blue solution as the stain. Carboxylated and sulfatic proteoglycans are selectively stained at a pH of 2.5. The differentiation between carboxyl and sulfate groups is only possible at pH 1.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)