47608

Formaldehyde solution

for molecular biology, BioReagent, ≥36.0% in H₂O (T)

• 동의어(들): Formic aldehyde, Formol, Formalin
• Linear Formula: HCHO
• CAS Number: 50-00-0
• Molecular Weight: 30.03
• Beilstein: 1209228
• MDL number: MFCD00003274
• UNSPSC 코드: 12352114
• PubChem Substance ID: 329757328
• NACRES: NA.52

속성

• Grade: for molecular biology
• Quality Level: 100
• vapor density: 1.03 (vs air)
• vapor pressure: 52 mmHg ( 37 °C)
• 제품 라인: BioReagent
• 형태: liquid
• autoignition temp.: 572 °F
• 포함: ~10% methanol as stabilizer
• 농도: ≥36.0% in H₂O (T); 36.0-38.0% (aldehyde method)
• 기술: electrophoresis: suitable
• 불순물: ≤0.03% free acid (as HCOOH)
• 무기 잔류물: ≤0.05% (as SO₄)
• pH: 2.8-4.0
• density: 1.09 g/mL at 20 °C; 1.09 g/mL at 25 °C (lit.)
• 음이온 미량물: chloride (Cl^-): ≤10 mg/kg; sulfate (SO₄^2-): ≤20 mg/kg
• 양이온 미량물: Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Na: ≤100 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Zn: ≤1 mg/mL
• λ: 2 M in H₂O
• UV 흡수: λ: 260 nm A_max: 0.01; λ: 280 nm A_max: 0.01
• 적합성: in accordance for electrophoresis of RNA in agarose gels
• 저장 온도: room temp
• SMILES string: [H]C([H])=O
• InChI: 1S/CH2O/c1-2/h1H2
• InChI key: WSFSSNUMVMOOMR-UHFFFAOYSA-N

설명

일반 설명

Formaldehyde is a molecular biology reagent that denaturates RNA during electrophoresis when incorporated in agarose gels.
It is an aliphatic aldehyde that is widely used in industrial applications for the synthesis of several chemicals such as melamine formaldehyde, urea-formaldehyde, phenol formaldehyde and industrial resins, paraformaldehyde, polyols, chelating agents, slow release fertilizers, 1,4-butanediol and hexamethylenetetramine.

애플리케이션

Formaldehyde solution is suitable for:

• denaturation and separation of RNA molecules
• anti-bacterial treatment
• fixation of cells

Formaldehydesolution has been used:

• in the fixation of human spermatogonial stem cell transplantation (SSCT).
• to crosslink T- cell acute lymphoblastic leukemia protein/DNA complex during chromatin immunoprecipitation.
• in fixing zebra fish larvae for neutrophil staining.

주의사항

may contain precipitate (paraformaldehyde)

원리

Formaldehyde disrupts the secondary structure of RNA and allows separation and identification of RNA species based on charge migration.

안전성 정보

• 픽토그램: GHS02,GHS06,GHS08,GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H226,H301 + H311,H314,H317,H330,H335,H341,H350,H370
• 예방조치 성명서: P202 - P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3
• 표적 기관: Eyes,Central nervous system, Respiratory system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point (°F): 133.0 °F - closed cup
• Flash Point (°C): 56.11 °C - closed cup
• 개인 보호 장비: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter