제품 이름
Lipase from Rhizopus niveus, powder (fine), ≥1.5 U/mg
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
biological source
fungus (Rhizopus niveus)
form
powder (fine)
specific activity
≥1.5 U/mg
mol wt
Mr ~83000
storage temp.
2-8°C
Quality Level
유사한 제품을 찾으십니까? 방문 제품 비교 안내
Application
Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester linkage at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet.
Biochem/physiol Actions
Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
General description
Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa.
Other Notes
1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from a triglyceride per minute at pH 7.7 and 40°C (olive oil as substrate)]; 300 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314, at pH 8.0 and 40°C as substrate.
Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.
Substrate specificity: preferentially hydrolyzes the 1- and 3-positions(middle to long fatty acids); optimum pH 5.0-7.0; optimum temperature 30-45°C; Selective enzymatic removal of protecting functions (C-terminal COOH function)
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Analysis of triacylglycerol accumulation under nitrogen deprivation in the red alga Cyanidioschyzon merolae
Masakazu Toyoshima
Microbiology (2016)
Purification, characterization and crystallization of two types of lipase from Rhizopus niveus
Mitsutaka Kohno
Bioscience, Biotechnology, and Biochemistry, 58(6) (1994)
Simulation of gastric lipolysis and prediction of felodipine release from a matrix tablet in the fed stomach
A.Diakidou
European Journal of Pharmaceutical Sciences (2009)
Y. H. Hui and George G. Khachatourians
Food Biotechnology (1995)
P. Braun et al.
Synlett, 105-105 (1990)
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.