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Merck
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Key Documents

P4832

Sigma-Aldrich

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt 150,000-300,000, solution, 0.01%, suitable for cell culture

동의어(들):

PLL Solution

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
12352202
eCl@ss:
32160406
NACRES:
NA.75

product name

Poly-L-lysine solution, mol wt 150,000-300,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture

무균

sterile-filtered

제품 라인

BioReagent

형태

solution

분자량

150,000-300,000

포장

pkg of 50 mL

농도

0.01%

기술

cell culture | mammalian: suitable

불순물

endotoxin, tested

solubility

water: soluble

배송 상태

ambient

저장 온도

2-8°C

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

L-lysine is a homopolypeptide having two enantiomeric forms, L and D lysine. Through its three reactive groups (the carboxylic acid and the α and ε amines), L-lysine has the capacity to generate various branched polymeric structures, including dendrimers, branched polylysine, and hyperbranched polylysine (PLL). Poly-L-lysine is a polymer made up of positively charged amino acid residues, with each lysine residue typically containing one HBr.

애플리케이션

Poly-L-lysine has been used:

  • In coating 96-well plates for cell attachment in testing respiratory burst activity.
  • In coating glass cover slips for immunofluorescent labeling of intracellular structures.
  • For pretreating 6-well plate in myosatellite cell isolation protocol.
  • In coating coverslips for cell adhesion in E14 neuron culture to study possible mechanism of action of circRNA_01477 in neurons.
Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

생화학적/생리학적 작용

Poly‐l‐lysine (PLL) acts as polycationic polymers that have been extensively used to carry drugs, genes etc. across the cell membrane. Poly-L-lysine (PLL) has the capability to engage in the formation of polyelectrolyte complexes (PECs) by interacting with anionic entities such as polyanions, nucleic acid chains and some drugs.
Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

성분

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

제조 메모

This product is supplied as a 0.01% sterile filtered solution in water, using a molecular weight of 150 - 300 kDa. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Modulating the poly-L-lysine structure through the control of the protonation?deprotonation state of L-lysine
Stagi L, et al.
Scientific Reports (2022)
Molecular mechanisms underlying oxytocin-induced cardiomyocyte protection from simulated ischemia?reperfusion
Gonzalez-Reyes A, et al.
Molecular and Cellular Endocrinology, 412, 170-181 (2015)
Modulating the poly-L-lysine structure through the control of the protonation-deprotonation state of L-lysine
Luigi Stagi, et al.
Scientific Reports, 12(1), 19719-19719 (2022)
Effects of sex steroids on indices of protein turnover in rainbow trout (Oncorhynchus mykiss) white muscle
Cleveland BM and Weber GM
General and Comparative Endocrinology, 174(2), 132-142 (2011)
Caitlin N Spaulding et al.
eLife, 7 (2018-01-19)
Uropathogenic E. coli (UPEC), which cause urinary tract infections (UTI), utilize type 1 pili, a chaperone usher pathway (CUP) pilus, to cause UTI and colonize the gut. The pilus rod, comprised of repeating FimA subunits, provides a structural scaffold for

문서

Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

프로토콜

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

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