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Merck
모든 사진(1)

주요 문서

P7280

Sigma-Aldrich

Poly-ᴅ-Lysine Hydrobromide

synthetic, mol wt 30,000-70,000, powder, γ-irradiated, suitable for cell culture, BioXtra

동의어(들):

PDL

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About This Item

Linear Formula:
D-Lys-(D-Lys)n-D-Lys · xHBr
CAS Number:
MDL number:
UNSPSC 코드:
12352202
PubChem Substance ID:
NACRES:
NA.75

제품명

Poly-D-lysine hydrobromide, average mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

생물학적 소스

synthetic (organic)

Quality Level

무균

γ-irradiated

제품 라인

BioReagent
BioXtra

양식

lyophilized powder

분자량

average mol wt 30,000-70,000

포장

pkg of 5 X 5 mg
pkg of 5 mg

농도

0.016—0.032 mmol lysine

기술

cell culture | mammalian: suitable

표면 범위

4 μg/cm2

solubility

H2O: soluble 50 mg/mL, clear, colorless

배송 상태

ambient

저장 온도

−20°C

SMILES string

O=C(C)[C@@](NC)([H])CCCCN.[Br]

InChI

1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H

InChI key

MEXAGTSTSPYCEP-UHFFFAOYSA-N

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일반 설명

Poly-D-Lysine Hydrobromide (PDL), a positively charged polymer consisting of lysine residues, where each lysine unit is associated with a hydrobromide molecule. This hydrobromide molecule gives the poly-D-lysine a unique characteristic, enabling it to form crystals and making it easily soluble in water-based solutions.

애플리케이션

Poly d lysine hydrobromide coating has been used:
  • In coating tissue culture plate wells and coverslips for immunofluorescence microscopy of primary neuronal culture to study neurological disorders associated with Zika virus infection.
  • To coat the coverslips for enhancing the attachment and proliferation of the myenteric cells isolated from the rat intestines.
  • In preparing surface of coverslips for cell attachment for studying the myenteric plexus of rat’s small intestine.
Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

생화학적/생리학적 작용

Poly-D-lysine (PDL) hydrobromide is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

특징 및 장점

Sterilized using γ-irradiation

성분

Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ß structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

주의사항

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

제조 메모

This product has a molecular weight of 30,000-70,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리 방문

Methods to Study the Myenteric Plexus of Rat Small Intestine
Hecking I, et al.
Cellular and Molecular Neurobiology, 43, 315?325-315?325 (2021)
Progesterone: A Neuroprotective Steroid of the Intestine
Stegemann LN, et al.
Cells, 12(8), 1206-1206 (2023)
Valentina Cigliola et al.
PloS one, 11(3), e0150880-e0150880 (2016-03-10)
Signalling through gap junctions contributes to control insulin secretion and, thus, blood glucose levels. Gap junctions of the insulin-producing β-cells are made of connexin 36 (Cx36), which is encoded by the GJD2 gene. Cx36-null mice feature alterations mimicking those observed
Zika virus infection of mature neurons from immunocompetent mice generates a disease-associated microglia and a tauopathy-like phenotype in link with a delayed interferon beta response
Manet C, et al.
Journal of Neuroinflammation, 19 (2022)
Nadine Dupuis et al.
Molecular pharmacology, 91(6), 595-608 (2017-03-21)
G protein-coupled receptors are the most important drug targets for human diseases. An important number of them remain devoid of confirmed ligands. GPR27 is one of these orphan receptors, characterized by a high level of conservation among vertebrates and a

문서

Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

프로토콜

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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