A9460

Annexin V from human placenta

≥90% (SDS-PAGE), buffered aqueous solution

• 동의어(들): Annexin V, Calphosbindin I, Lipocortin V, PAP-1
• UNSPSC 코드: 41106305
• NACRES: NA.32

속성

• 생물학적 소스: human placenta
• Quality Level: 200
• 분석: ≥90% (SDS-PAGE)
• 형태: buffered aqueous solution
• 분자량: 33 kDa (four subunits)
• 기술: cell based assay: suitable
• 불순물: Human Source(HCV), tested negative
• solubility: water: soluble
• 적합성: suitable for molecular biology
• UniProt 수납 번호: P08758
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... ANXA5(308)

설명

일반 설명

Research area: Apoptosis

Annexin V is a calcium-dependent phospholipid-binding protein. It is expressed in high concentration in cells with barrier functions, such as vascular endothelium, epithelial cells in bile ducts, placental trophoblasts, mammary ducts, renal tubules, and nasal epithelium.

애플리케이션

Annexin V from human placenta has been used:

• to verify the involvement of phosphatidylserine in adhesion using flow cytometry
• to test adhesion specificity of phosphatidylserine
• for synthesis of NIR700-Annexin-V imaging probe
• in dynamic light scattering (DLS) assay to test the effects of various treatments on virion aggregation

생화학적/생리학적 작용

Annexins are ubiquitous homologous proteins that bind phospholipids in the presence of calcium. The cellular changes involved in the apoptotic process include loss of phospholipid asymmetry during the early stages. This phenomenon is universal and is not limited to stimulus or to mammalian cells, but also occurs in insect and plant cells. In living cells, phosphatidylserine is transported to the inside of the lipid bilayer by the Mg²⁺ATP dependent enzyme, aminophospholipid translocase. At the onset of apoptosis, phosphatidylserine becomes translocated to the external surface of the cell membrane. Since the movement of phosphatidylserine from the internal membrane surface to the external surface is an early indicator of apoptosis, annexin V and its conjugates that interact strongly and specifically with phosphatidylserine may be used to detect apoptosis. Annexin V conjugates can be used to detect apoptotic cells significantly earlier than DNA-based assays. Fluorescent dye labeled annexin V have applications in flow cytometry, fluorescence microscopy, and laser scanning cytometry.

It is a widely used marker for identifying apoptotic cells due to its high affinity for phosphatidylserine, which is normally located on the inner leaflets of membranes but moves to the cell surface during programmed cell death.

포장

Package size based on protein content

물리적 형태

Solution in 40 mM Tris-HCl, pH 7.5, containing 150 mM NaCl, 1 mM DTT and 0.05% sodium azide.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable