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Merck
모든 사진(1)

주요 문서

C1104

Sigma-Aldrich

Monoclonal Anti-Caspase 7 antibody produced in rat

clone 11E4, purified immunoglobulin, buffered aqueous solution

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

rat

Quality Level

결합

unconjugated

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

11E4, monoclonal

양식

buffered aqueous solution

분자량

antigen (full length) 35 kDa
antigen (subunit) 20 kDa

종 반응성

human

기술

immunoprecipitation (IP): suitable
microarray: suitable
western blot: 2-4 μg/mL using a whole extract of cultured human acute T cell leukemia Jurkat cells

동형

IgG2a

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... CASP7(840)

일반 설명

Caspase-7 is a apoptosis-related cysteine peptidase, commonly known as CASP7. It is a human protein encoded by the CASP7 gene. CASP7 is a member of the caspase (cysteine aspartate protease) family of proteins and is an executioner protein of apoptosis.

면역원

recombinant p20 subunit of human caspase-7.

애플리케이션

Monoclonal Anti-Caspase 7 antibody produced in rat is suitable for the following applications:
  • Immunoprecipitation
  • Microarray
  • Western blotting at a concentration of 2-4μg/mL using a whole extract of cultured human acute T cell leukemia Jurkat cells.

생화학적/생리학적 작용

Caspase-3 and -7 plays a central role in the execution-phase of cell apoptosis. The gene for caspase 7 (also known as Mch3, CMH-1 and ICELAP3) encodes a “short” prodomain protein product of 35 kDa that belongs to the Ced3/ICE family of cysteine proteases. Caspase 7 undergoes acleavage upon activation by caspase-3, -9 and -10, and granzyme B, leading to p20 and p12 subunits. It is an important intracellular effector of granzyme B-mediated apoptosis and cytotoxic Tlymphocyte- induced cell killing in vivo. Monoclonal antibodies reacting specifically with caspase 7 are useful tools for the study of protease networks involved in development and regulation and governing the life and death of cells and tissues.

제조 메모

The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids


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문서 라이브러리 방문

A M Chinnaiyan et al.
Current biology : CB, 6(7), 897-899 (1996-07-01)
Cytotoxic T lymphocytes (CTLs) and natural killers (NK) cells provide immune surveillance against viruses and neoplasms, and play a central role in the pathogenesis of autoimmune disease, AIDS and graft rejection. Thus, it is important to understand the precise molecular
Witold A Witkowski et al.
Protein science : a publication of the Protein Society, 20(8), 1421-1431 (2011-06-16)
Caspases are a powerful class of cysteine proteases. Introduction of activated caspases in healthy or cancerous cells results in induction of apoptotic cell death. In this study, we have designed and characterized a version of caspase-7 that can be inactivated
Yin Guo et al.
The Journal of biological chemistry, 277(16), 13430-13437 (2002-02-08)
Caspase-2 is one of the earliest identified caspases, but the mechanism of caspase-2-induced apoptosis remains unknown. We show here that caspase-2 engages the mitochondria-dependent apoptotic pathway by inducing the release of cytochrome c (Cyt c) and other mitochondrial apoptogenic factors
E Svandova et al.
Cell death & disease, 5, e1366-e1366 (2014-08-15)
Caspase-3 and -7 are generally known for their central role in the execution of apoptosis. However, their function is not limited to apoptosis and under specific conditions activation has been linked to proliferation or differentiation of specialised cell types. In
H Duan et al.
The Journal of biological chemistry, 271(3), 1621-1625 (1996-01-19)
Members of the ICE/ced-3 gene family have been implicated as components of the cell death pathway. Based on similarities with the structural prototype interleukin-1 beta-converting enzyme (ICE), family members are synthesized as proenzymes that are proteolytically processed to form active

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