C3389

Acetylcholinesterase from Electrophorus electricus (electric eel)

Type VI-S, lyophilized powder, 200-1,000 units/mg protein

• 동의어(들): AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
• CAS Number: 9000-81-1
• 효소 위원회 번호: 3.1.1.7 (BRENDA, IUBMB)
• EC Number: 232-559-3
• MDL number: MFCD00081268
• UNSPSC 코드: 12352204
• NACRES: NA.77

속성

• 유형: Type VI-S
• 형태: lyophilized powder
• 특이 활성도: 200-1,000 units/mg protein
• 분자량: 280 kDa
• 구성: Protein, ≥45% biuret
• solubility: 20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.)
• 응용 분야: diagnostic assay manufacturing
• 저장 온도: −20°C

설명

일반 설명

Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E^1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

애플리케이션

The enzyme from sigma has been used as a reference to to evaluate the effect of aspartame metabolites on hippocampal acetylcholinesterase activity. The enzyme has also been used in immobilization studies for the rapid detection of acetylthiocholine chloride.

생화학적/생리학적 작용

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H₂O to choline + acetic acid.

단위 정의

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

물리적 형태

Lyophilized powder containing Tris buffer salts

제조 메모

This enzyme dissolves in 20 mM Tris-HCl buffer, pH 7.5 at 1 mg/mL concentration, yielding a clear solution.

분석 메모

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)