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Key Documents

C4407

Sigma-Aldrich

Human Collagen Type III

from human placenta, powder, suitable for cell culture

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
12352202
NACRES:
NA.32

product name

Collagen from human placenta, Bornstein and Traub Type III (Sigma Type X), powder

생물학적 소스

human placenta

Quality Level

분석

≥90% (GE)

형태

powder

기술

cell culture | mammalian: suitable

불순물

HIV, hepatitis B and hepatitis C, none detected

solubility

aqueous acid: soluble

UniProt 수납 번호

저장 온도

2-8°C

유전자 정보

human ... COL3A1(1300)

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애플리케이션

Collagen from human placenta has been used to assess the bioelectric effects of quinine on airway epithelial cells, to study the selective toxicity of engineered lentvirus lytic peptides and in a particle aggregation assay for the rapid detection of fibronectin, fibrinogen and collagen receptors on Staphylococcus aureus. It has also been used in a magnesium-dependent, collagen-binding assay during characterization of human lung tumor-associated antigens.

성분

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue.

주의사항

This product should be stored at 2-8 °C and will retain activity in these conditions for 2 years.

제조 메모

This product was prepared by a modification of the pepsin extraction and salt fractionation method of Hill, R.J. and Harper, E., Anal. Biochem., 141 (1984). It is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C.

분석 메모

An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.

기타 정보

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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