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CAT100

Sigma-Aldrich

Catalase Assay Kit

sufficient for ≥100 tests enzymatic, determination of catalase activity in tissues and cells

동의어(들):

Catalase Activity Detection Kit

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크기 선택

W441330-SAMPLE-K
$187.00
100 G
$312.00
1 KG
$1,120.00

$187.00

Check Cart for Availability
벌크 견적 요청
모든 사진(1)

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보기 변경
W441330-SAMPLE-K
$187.00
100 G
$312.00
1 KG
$1,120.00

About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

$187.00

Check Cart for Availability
벌크 견적 요청

Quality Level

200

사용

sufficient for ≥100 tests enzymatic

검출 방법

colorimetric

배송 상태

wet ice

저장 온도

2-8°C

유전자 정보

human ... CAT(847)

관련 카테고리

유사한 품목 비교

전체 비교 보기

차이점 표시

1 of 4

이 품목
W336408W256501W292516
trans-3-Hexenyl acetate ≥95%, stabilized, FG

W441330

trans-3-Hexenyl acetate

Methyl trans-3-hexenoate ≥97%, FG

W336408

Methyl trans-3-hexenoate

Hexyl acetate ≥98%, FCC, FG

W256501

Hexyl acetate

Propyl acetate natural, ≥97%, FCC, FG

W292516

Propyl acetate

grade

FG, Kosher, Halal

grade

FG, Fragrance grade, Halal, Kosher

grade

FG, Fragrance grade, Halal, Kosher

grade

FG, Fragrance grade, Halal, Kosher, natural

organoleptic

banana; green; fruity; pear; rum

organoleptic

green; fruity

organoleptic

apple; green; fruity; pear; sweet

organoleptic

celery; fruity; pear

food allergen

no known allergens

food allergen

no known allergens

food allergen

no known allergens

food allergen

no known allergens

documentation

see Safety & Documentation for available documents

documentation

see Safety & Documentation for available documents

documentation

see Safety & Documentation for available documents

documentation

see Safety & Documentation for available documents

agency

follows IFRA guidelines, meets purity specifications of JECFA

agency

follows IFRA guidelines, meets purity specifications of JECFA

agency

follows IFRA guidelines, meets purity specifications of JECFA

agency

follows IFRA guidelines, meets purity specifications of JECFA

assay

≥95%

assay

≥97%

assay

≥98%

assay

≥97%

일반 설명

The Catalase Assay Kit contains all necessary components for studying catalase activity in various tissues and subcellular organelles.

애플리케이션

Sutitable for Colorimetric and UV Assays

생화학적/생리학적 작용

Catalase is a ubiquitous antioxidant enzyme which catalyses the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Hydrogen peroxide is formed in the eukaryotic cell as a by-product of various oxidases and superoxide dismutases. Hydrogen peroxide accumulation in cells causes oxidation of cellular targets such as DNA, proteins, and lipids leading to mutagenesis and cell death. Removal of the H2O2 from the cell by catalase provides protection against oxidative damage to living cells and its role in oxidative stress related diseases has been widely studied.

특징 및 장점

  • Useful for determining catalase activity - may be used in various tissues and cells
  • Simple, optimized protocol - A simple colorimetric assay for analysis of peroxisome enrichment and catalase activity

적합성

Suitable for studying catalase activity in various tissues and subcellular organelles.

원리

This assay method is based on the measurement of the hydrogen peroxide substrate remaining after the action of catalase. First, the catalase converts hydrogen peroxide to water and oxygen (catalatic pathway) and then this enzymatic reaction is stopped with sodium azide. An aliquot of the reaction mix is then assayed for the amount of hydrogen peroxide remaining by a colorimetric method.10 The colorimetric method uses a substituted phenol (3,5-dichloro-2-hydroxybenzenesulfonic acid), which couples oxidatively to 4-aminoantipyrine in the presence of hydrogen peroxide and horseradish peroxidase (HRP) to give a red quinoneimine dye (N-(4-antipyryl)-3-chloro-5-sulfonatep-benzoquinone-monoimine) that absorbs at 520 nm

단위 정의

One unit of catalase will decompose 1.0 micromole of hydrogen peroxide to oxygen and water per minute at pH 7.0 at 25 °C at a substrate concentration of 50 mM hydrogen peroxide.

제조 메모

Use ultrapure water in preparation of all solutions.

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS
  • P6782Peroxidase from horseradish, Type VI-A, essentially salt-free, lyophilized powder, 950-2000 units/mg solid (using ABTS), ≥250 units/mg solid (using pyrogallol) 5 mgSDS
  • 323381Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H2OSDS

픽토그램

Health hazard
GHS08

신호어

Danger

유해 및 위험 성명서

H334

예방조치 성명서

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

    • 가장 최신 버전 중 하나를 선택하세요:

    시험 성적서(COA)

    Lot/Batch Number
    0000389380
    0000389380
    0000198880
    0000198880
    0000160559

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    COA 검색

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    문서 라이브러리 방문

    이미 열람한 고객

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    M E Tome et al.
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    Glucocorticoids are used for the treatment of lymphoid neoplasms, taking advantage of the well-known ability of these compounds to cause apoptosis in lymphoid tissues. Previously, we have shown that dexamethasone, a synthetic glucocorticoid, causes a down-regulation of several antioxidant defense
    S Tada-Oikawa et al.
    FEBS letters, 442(1), 65-69 (1999-01-29)
    Pulsed field gel electrophoresis showed that the initiation time of DNA breakage induced by the DNA alkylating agent duocarmycin A, which is not a redox-cycling agent, was almost the same in the human leukemia cell line HL-60 and its H2O2-resistant
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    The involvement of reactive oxygen species in Ca(2+)-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2, 4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae
    M B Hampton et al.
    FEBS letters, 414(3), 552-556 (1997-10-10)
    The induction of apoptosis in Jurkat T-lymphocytes with 50 microM hydrogen peroxide was associated with caspase activation. Caspase activity was first detected 3 h after treatment, and the morphological features of apoptosis were apparent by 6 h. At higher concentrations
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    문서

    Organelle Isolation

    Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

    Mitochondrial Stress and ROS

    Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

    자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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