콘텐츠로 건너뛰기
Merck
모든 사진(2)

문서

CS1000

Sigma-Aldrich

Superoxide Anion Assay Kit

sufficient for 100 assays (96 well plates)

동의어(들):

Superoxide Detection Kit

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 100 assays (96 well plates)

Quality Level

배송 상태

wet ice

저장 온도

2-8°C

애플리케이션

The Superoxide Anion assay kit provides a useful qualitative cell-based assay for the measurement of O2 anion status in cells. The kit can be used to test changes in superoxide anion levels following oxidative stress directly in whole cells. In addition, the kit can be also used for the detection of superoxide dismutase (SOD) activity. This activity was tested on erythrocytes, leucocytes, and plasma.

생화학적/생리학적 작용

The superoxide anion (O2-) is a short-lived radical generated by the addition of an electron to oxygen. It is formed in response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and γ-radiation, or by oxidases like xanthine oxidase or NADPH oxidase. Once formed, O2- attacks cellular components causing damage to lipids, proteins, and DNA. This can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders.

분석 메모

The kit method is based on the oxidation of luminol by superoxide anions resulting in the formation of chemiluminescence light. This method utilizes a specific, non-toxic enhancer that amplifies the chemiluminescent signal.

키트 구성품 전용

제품 번호
설명

  • Assay Buffer 25 mL

  • Assay Medium 20 mL

  • Enhancer Solution, 40 mM 500 μL

  • Luminol Solution 500 μL

  • Xanthine Solution 500 μL

  • Xanthine Oxidase .5 U

  • Superoxide Dismutase from bovine liver, 4 units/μL 400 U

Storage Class Code

10 - Combustible liquids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

이미 열람한 고객

Muthusamy V Shanmuganathan et al.
Microbes and infection, 16(2), 134-141 (2013-10-29)
Macrophages serve as permissive niches for Escherichia coli (E. coli) K1 to attain high grade bacteremia in the pathogenesis of meningitis in neonates. Although pterin levels are a diagnostic marker for immune activation, the role of macrophages in pterin production
Wen-Bin Zhang et al.
Oxidative medicine and cellular longevity, 2021, 6610543-6610543 (2021-02-06)
Doxorubicin- (DOX-) related cardiac injury impairs the life quality of patients with cancer. This largely limited the clinical use of DOX. It is of great significance to find a novel strategy to reduce DOX-related cardiac injury. Oroxylin A (OA) has
Dawei Jiang et al.
Nature biomedical engineering, 2(11), 865-877 (2018-12-07)
Patients with acute kidney injury (AKI) frequently require kidney transplantation and supportive therapies, such as rehydration and dialysis. Here, we show that radiolabelled DNA origami nanostructures (DONs) with rectangular, triangular and tubular shapes accumulate preferentially in the kidneys of healthy
Erythropoietin activates nitric oxide synthase in murine erythrocytes
Mihov,D et al.
The American Journal of Physiology, 279(2), C378-C388 (2009)
Hannah Watson et al.
The Journal of experimental biology, 221(Pt 13) (2018-05-12)
In oviparous animals, maternally transferred antioxidants protect the embryo from oxidative damage from high rates of reactive oxygen species (ROS) production incurred by rapid growth. Elevated ROS exposure, beyond that incurred by normal growth, can occur as a result of

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.