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Merck
모든 사진(1)

주요 문서

D6546

Sigma-Aldrich

DMEM - high glucose

With sodium Bicarbonate, sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

동의어(들):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.75

제품명

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

무균

sterile-filtered

양식

liquid

기술

cell culture | mammalian: suitable

불순물

endotoxin, tested

성분

NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
HEPES: no
L-glutamine: no
glucose: high

배송 상태

ambient

저장 온도

2-8°C

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일반 설명

This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

애플리케이션

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

재구성

Supplement with 0.584 g/L L-glutamine.

픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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Cell culture protocol for passaging and splitting suspension cell lines

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