추천 제품
Quality Level
사용
sufficient for 750 assays (2 ml each)
기술
nucleic acid detection: suitable
저장 온도
−20°C
일반 설명
The kit has all the reagents necessary for quantitation of DNA using the fluorescent dye bisBenzimide (Hoechst 33258). The kit allows highly sensitive, quick, and accurate determinations of double-stranded DNA from 10ng/ml to 5μg/ml, even in the presence of RNA and proteins. Fluorometry is a highly sensitive and simple method used for DNA quantitation. The fluorescent dye, bisBenzimide H 33258, which binds primarily to AT sequences in the minor groove of double-stranded DNA (dsDNA), is specific for quantitation of nanogram amounts of DNA (10 ng/ml to 10 mg/ml). When excited at 360 nm, the fluorescence emission at 460 nm of the dye increases significantly in the presence of DNA.
애플리케이션
DNA Quantitation Kit, Fluorescence Assay has been used to measure the concentration/content of DNA:
- in neonatal rat ventricular myocytes (NRVMs)
- in human mesenchymal stem cells (hMSCs)/samples for normalization of the relative alkaline phosphatase (ALP) amount
- in mouse embryonic fibroblast cell line (NIH3T3 cells)
특징 및 장점
- Specific for the quantitation of nanogram amounts of DNA
- Works well with purified preparations as well as with DNA from crude extracts containing RNA and proteins
키트 구성품 전용
제품 번호
설명
- Detailed technical bulletin 1 ea
관련 제품
제품 번호
설명
가격
Storage Class Code
10 - Combustible liquids
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
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