Skip to Content
MilliporeSigma
All Photos(1)

Documents

DTT-RO

Roche

DTT

crystalline powder, =97% (Ellman′s reagent), Mr 154.3

Sign Into View Organizational & Contract Pricing

Synonym(s):
DL-Dithiothreitol, 1,4-dithiothreitol, cleland′s reagent, dithiothreitol, 1,4-, threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
Linear Formula:
HSCH2CH(OH)CH(OH)CH2SH
CAS Number:
Molecular Weight:
154.25
Beilstein/REAXYS Number:
1719757
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:

description

1,4-Dithiothreitol

assay

97% (Ellman′s reagent)

form

crystalline powder

mol wt

Mr 154.3

reaction suitability

reagent type: reductant

packaging

pkg of 10 g (10708984001)
pkg of 2 g (10197777001)
pkg of 25 g (11583786001)

manufacturer/tradename

Roche

pH

5.1 (20 °C)

mp

41-44 °C (lit.)

storage temp.

2-8°C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

Looking for similar products? Visit Product Comparison Guide

General description

1,4-dithiothreitol is commonly known as Cleland′s reagent. It confers protection to thiol groups and reduces disulfide bonds in peptides and proteins. It reduces disulfide bonds to sulfhydryl group and is commonly used in receptor studies, to determine the functional importance of disulfide bonds during receptor occupancy and functionality. It also maintains monothiols completely in the reduced state.

Application

1,4-dithiothreitol is used in:
  • Isolation, purification and characterization of proteins and enzymes
  • Measurement of enzyme activities (reactivation of enzymes)
  • Determination of disulfide groups in proteins and enzymes
  • DNA extraction prior to amplification
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Specifications

Oxidized form: <2.5% (absorbance at 283nm)

Sequence

Enantiomers
Our DTT-preparation is optically inactive, i.e. it is the D,L-DTT.

Preparation Note

Storage conditions (working solution): A solution of DTT in Hepes buffer, pH 7.75 is stable for one week at 2 to 8 °C if the container is tightly sealed and the solution is protected from atmospheric oxygen by argon or nitrogen.

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Synthesis of optically active Cleland's reagent [(-)-1, 4-dithio-L-threitol]
Carmack M and Charles J K
The Journal of Organic Chemistry, 33(5), 2171-2173 (1968)
Emanuelle Pascolo et al.
The Journal of biological chemistry, 277(18), 15566-15572 (2002-02-21)
Telomerase, a ribonucleoprotein acting as a reverse transcriptase, has been identified as a target for cancer drug discovery. The synthetic, non-nucleosidic compound, BIBR1532, is a potent and selective telomerase inhibitor capable of inducing senescence in human cancer cells (). In
Frans J L Robberts et al.
Journal of clinical microbiology, 42(4), 1505-1510 (2004-04-09)
Sequence analysis of Pneumocystis jiroveci internal transcribed spacer (ITS) regions has become an important epidemiological tool. The objectives of the present study were to investigate sequence variations in the ITS1-5.8S ribosomal DNA (rDNA)-ITS2 regions; determine the P. jiroveci genotypes present
J Donaldson et al.
British journal of pharmacology, 87(1), 191-199 (1986-01-01)
1,4-Dithiothreitol (DTT; 1 mM, 30 min preincubation) produced a small, non-specific potentiation of spasmogenic activity in longitudinal muscle strips of guinea-pig small intestine. A direct comparison of contractile responses elicited by histamine and a range of H1- and non-H1-receptor agonists

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service