E9644

hEGF

EGF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 동의어(들): Epidermal Growth Factor human, EGF
• CAS Number: 62253-63-8
• MDL number: MFCD00132565
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 생물학적 소스: human
• Quality Level: 300
• 재조합: expressed in E. coli
• 분석: ≥97% (SDS-PAGE)
• 형태: lyophilized powder
• 효능: 0.08-0.8 ng/mL ED₅₀/EC₅₀
• 분자량: ~6 kDa
• 포장: pkg of 5X0.2 mg; pkg of 0.2 mg; pkg of 0.5 mg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: ≤1 EU/μg endotoxin (EGF)
• 색상: white
• solubility: water: soluble, clear, colorless
• UniProt 수납 번호: P01133
• 저장 온도: −20°C
• 유전자 정보: human ... EGF(1950)

설명

애플리케이션

This product is used as a mitogen, triggering the cell to commence mitosis, in a variety of cell lines. In tissue cultures, EGF acts to reduce or eliminate the requirement for serum and can be used in conjunction with other media additives and hormones.

생화학적/생리학적 작용

Epidermal Growth Factor (EGF) is a small mitogenic polypeptide present throughout a large number of tissues and body fluids in many mammalian species. EGF is a member of a growth factor family characterized by 6 conserved cysteine motifs that form three disulfide bonds. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, vascular and corneal endothelial cells, bovine granulosa, HeLa cells, SV40-3T3 cells and mammary epithelial cells.
Cellular functions affected by EGF: mitosis, ion flux, glucose transport, glycolysis, nucleic acid and protein synthesis, survival, growth, proliferation and differentiation.
Biological affects of EGF: inhibition of gastric acid secretion, fetal growth and development and neuromodulation in the central nervous system.
Pathways affected by EGF: EGFR signaling, MAPK cascade, PIP, Ca+2 signaling

성분

Human EGF (hEGF) is an identical molecule to β-urogastrone, a molecule isolated on the basis of its ability to inhibit gastric acid secretion. EGF is structurally homologous to human transforming growth factor-α, and both exert their actions through EGF receptors. E9644 is the N-terminal methionyl form of natural mature EGF.

주의사항

This product should be stored at -20°C and will retain activity for two years. After reconstitution, it can be stored at 2-8°C for one month, or frozen in aliquots at -70°C or -20°C for longer periods.

제조 메모

This product was lyophilized from a 0.2 μm-filtered solution of phosphate buffered saline, at pH 7.4. It should be reconstituted by adding the contents of the vial to 1 mg/mL using 10 mM acetic acid. To dilute to lower concentrations, but no fewer than 10 μg/mL, an addition of 0.1% BSA or HSA will be needed. For use in proliferation assays, further dilute the sample with medium without albumin.

분석 메모

The biological activity of this product is measured in a proliferation assay using BALB/MK cells. The EC₅₀ is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)