F0635

Rat Fibronectin

from rat plasma, powder, suitable for cell culture

• 동의어(들): Fibronectin
• EC Number: 289-149-2
• MDL number: MFCD00131062
• UNSPSC 코드: 12352202
• eCl@ss: 42020141
• NACRES: NA.75

속성

• product name: Fibronectin rat plasma, powder, BioReagent, suitable for cell culture
• 생물학적 소스: rat plasma
• Quality Level: 200
• 무균: sterile
• 제품 라인: BioReagent
• 형태: powder
• 포장: pkg of 0.5 mg; pkg of 1 mg; pkg of 2 mg
• 기술: cell culture | mammalian: suitable
• 표면 범위: 1‑5 μg/cm²
• solubility: H₂O: 1 mg/mL at 37 °C, clear, colorless (some particles)
• UniProt 수납 번호: P04937
• 결합 특이성: Peptide Source: Collagen
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: rat ... Fn1(25661)

설명

일반 설명

Fibronectin (FN)/FN1 (fibronectin 1) is a multifunctional glycoprotein, produced by hepatic stellate cells (HSCs). It is a constituent of extracellular matrix (ECM). FN1 is found in the cell membrane and cytoplasm. It is also synthesized and liberated by fibroblasts, endothelial cells, macrophages and hepatocytes in vivo.

애플리케이션

Fibronectin rat plasma has been used in the induction of differentiation , adhesion to extracellular matrix, neural differentiation and primary neuronal ventral mesencephalon cultures.

생화학적/생리학적 작용

Fibronectin (FN)/FN1 (fibronectin 1) plays a major role in cell cycle progression, cell adhesion and proliferation and fibrotic progression. It is essential for for collagen matrix assembly. High expression of FN has a vital role during liver fibrosis. FN1 helps in the support of other matrix proteins.

성분

Fibronectin is an adhesive glycoproteins found in both the cell surface and in plasma.

주의사항

The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.

제조 메모

This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, showing a single arc, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.

분석 메모

A single arc is shown when reacted with anti-fibronectin by IEP (immunoelectrophoresis).

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)