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Merck
모든 사진(1)

주요 문서

F2442

Sigma-Aldrich

Fetal Bovine Serum

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

동의어(들):

Fetal Bovine Sera, Fetal Calf Serum, FBS, FCS, sera, serum

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About This Item

MDL number:
UNSPSC 코드:
12352207
eCl@ss:
32160409
NACRES:
NA.71
생물학적 소스:
bovine fetus
기원:
USA origin
무균:
sterile-filtered
배송 상태:
dry ice

생물학적 소스

bovine fetus

Quality Level

무균

sterile-filtered

제품 라인

FBS Classic

구성

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

기원

USA origin

기술

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

불순물

≤10 EU/mL endotoxin

배송 상태

dry ice

저장 온도

−20°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Fetal Bovine Serum (FBS) is a complex mixture with low and high molecular weight biomolecules exhibiting optimal growth-enhancing and growth-suppressing functions. FBS contains molecules, such as growth factors, proteins, vitamins, trace elements, hormones etc. These molecules are important for the growth and maintenance of cells. It is a common supplement for cell culture media. FBS is derived from the calf blood. It is devoid of fibrin and clotting factors. Heat inactivation of FBS blocks the complement system. FBS derived from clotted blood is the most widely used undefined supplement in eukaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM), Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM), M199 and Roswell Park Memorial Institute medium, RPMI-1640.

애플리케이션

Fetal Bovine Serum has also been used as a component of:
  • perivascular-resident macrophage-like melanocytes (PVM/M) growth medium
  • synthetic oviduct fluid (SOF)
  • Eagle′s minimum essential medium (EMEM)
  • neurobasal medium
  • Hyclone McCoys 5 A medium
  • Hanks medium

생화학적/생리학적 작용

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

분석 메모

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

기타 정보

Tested for the presence of bacteria, virus, mycoplasma, endotoxin and for hemoglobin content. Triple filtered with 0.1 micron membrane under aseptic conditions.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves


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시험 성적서(COA)

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Axenic Procyclic Culture of L. tropica and L. donovani in Culture of FBS-free Medium
Mosa FM, et al.
Ife Journal of Science, 4B, 3088-3093 (2015)
MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member.
Grigoryev SA et al.
The Journal of Biological Chemistry, 274, 5626-5626 (1999)
Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.
van der Valk J et al.
Toxicology in vitro, 24, 1053-1053 (2010)
Cunzhi Yu et al.
Frontiers in pharmacology, 8, 832-832 (2017-12-07)
Low solubility, tissue accumulation, and toxicity are chief obstacles to developing triptolide derivatives, so a better understanding of the pharmacokinetics and toxicity of triptolide derivatives will help with these limitations. To address this, we studied pharmacokinetics and toxicity of (5R)-5-hydroxytriptolide

문서

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Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

프로토콜

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

관련 콘텐츠

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Step-by-step protocol for generating apical-out human gut organoids for microbiome, ADME/Tox, viral and gastrointestinal related disease research. See the complete organoid culture protocol.

머크의 포괄적인 우태혈청(FBS) 포트폴리오에서 귀하의 실험실 요건과 예산에 적합한 제품을 찾아보십시오. 지구촌의 문제가 FBS 가격에 미치는 영향에 대해 알아보십시오.

Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.

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