F2653

Anti-Mouse IgG (Fab specific) F(ab′)₂ fragment–FITC antibody produced in goat

flow cytometry grade, affinity isolated antibody, buffered aqueous solution

• MDL number: MFCD00162622
• UNSPSC 코드: 12352203
• NACRES: NA.46

속성

• 생물학적 소스: goat
• Quality Level: 200
• 결합: FITC conjugate
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: secondary antibodies
• Grade: flow cytometry grade
• 클론: polyclonal
• 형태: buffered aqueous solution
• 기술: flow cytometry: 1:100; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

IgG, a monomer, is the principal antibody circulating in blood which assist in phagocytic destruction of foreign microorganism and is the only class of Ig that can cross the placenta in humans. Cell-sorting done by differential binding of anti-mouse IgG (Fab Specific) - FITC antibody separates tumour cells from macrophages. This antibody can be used to incubate somatic cell nuclear transfer (SCNT) rat embryos for immunocytochemical observation. It also can be used as a secondary antibody to study the dynamics of activated B lymphocytes in small intestine, Peyer′s patch germinal center as well as non-germinal center, spleen and the mesenteric lymph node. Anti-mouse IgG antibody reacts specifically with all mouse immunoglobulins.

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

특이성

Binds all mouse Igs.
Useful when trying to avoid background staining due to the presence of Fc receptors.

면역원

Purified Fab fragments of mouse IgG.

애플리케이션

Anti-Mouse IgG (Fab Specific) FITC Conjugate antibody is used in traditional method of immunocytochemical assessment of ZAP-70 expression. It can also be used in immunocytochemical observation of the SCNT embryos.

Anti-Mouse IgG (Fab specific) F(ab′)2 fragment-FITC antibody produced in goat has been used in:

• indirect immunofluorescence staining
• immunofluorescence staining
• flow cytometry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

생화학적/생리학적 작용

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

기타 정보

Antibody adsorbed with bovine, equine and human serum proteins.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

제조 메모

Adsorbed to reduce background staining with bovine, horse or human samples.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)