추천 제품
생물학적 소스
(Bioreactor)
Quality Level
결합
unconjugated
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
M1, monoclonal
양식
buffered aqueous solution
정제법
using Protein A
종 반응성
all
농도
2-5 mg/mL
기술
western blot: 10 μg/mL
면역원 서열
DYKDDDDK
배송 상태
dry ice
저장 온도
−20°C
일반 설명
The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.
Method of purification - Protein A
Method of purification - Protein A
면역원
FLAG; peptide sequence DYKDDDDK
애플리케이션
Monoclonal ANTI-FLAG® M1 antibody is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunofluorescence (1 paper)
Learn more product details in our FLAG® applications portal.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunofluorescence (1 paper)
Learn more product details in our FLAG® applications portal.
생화학적/생리학적 작용
Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca2+-dependent; the complex dissociates in the absence of calcium ions.
물리적 형태
Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).
제조 메모
Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.
법적 정보
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
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