F5387

Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• 동의어(들): Goat Anti-Mouse IgG (Fc specific)–Fluorescein isothiocyanate
• MDL number: MFCD00162628
• UNSPSC 코드: 12352203
• NACRES: NA.46

속성

• 생물학적 소스: goat
• Quality Level: 200
• 결합: FITC conjugate
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: secondary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 저장 조건: protect from light
• 기술: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100; indirect immunofluorescence: 1:32
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

IgG subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

특이성

Goat polyclonal anti-Mouse IgG (Fc specific)–FITC antibody detects all mouse IgG subclasses, it shows no reactivity with mouse IgG Fab fragment or human IgG. Identity and purity of the antibody is established by immunoelectrophoresis, prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion against anti-goat IgG and anti-goat whole serum result in single arcs of precipitation. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC.

면역원

Purified mouse IgG Fc fragment

애플리케이션

Anti-Mouse IgG (Fc specific)-FITC antibody has be used for

• Immunofluorescence
• Immunohistochemistry
• Immunostaining
• Confocal microscopy
• Flow cytometry

생화학적/생리학적 작용

IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections IgG is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Anti-Mouse IgG (Fc specific)-FITC antibody is specific for Fc fragment of all mouse IgG subclasses. It shows no reactivity with mouse IgG Fab fragment or human IgG. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC.

기타 정보

Antibody adsorbed with human IgG.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

제조 메모

Adsorbed to reduce background with human samples.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)