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Merck
모든 사진(3)

문서

G5637

Sigma-Aldrich

Giemsa stain

certified by the Biological Stain Commission, powder or crystals

동의어(들):

Azure mixture sicc. Giemsa stain

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About This Item

실험식(Hill 표기법):
C14H14ClN3S
CAS Number:
Molecular Weight:
291.80
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Giemsa stain, certified by the Biological Stain Commission

Grade

certified by the Biological Stain Commission

형태

powder or crystals

색상

dark green to very dark green

mp

300 °C (lit.)

solubility

ethanol: 1 mg/mL, blue to very deep blue

흡수

≥0.6 at 647-653 nm in methanol at 0.005 g/L

응용 분야

diagnostic assay manufacturing
hematology
histology

저장 온도

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

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일반 설명

Giemsa is a versatile polychromatic stain, which is suitable for staining a diverse range of specimens. It is a buffered thiazine-eosinate solution designed to provide coloration of blood cells. Giemsa’s stain is frequently used for diagnostic purposes in hematology to differentiate the nuclear and cytoplasmic morphology of platelets, RBCs, WBCs, and parasites. It is frequently used in combination with other dye solutions as May-Grunwald’s solution for Pappenheim (MGG) and Wright-Giemsa. The Giemsa stain was adapted to histology due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. Giemsa′s staining solution is composed of methylene blue, azure, and eosin.

애플리케이션

Giemsa stain has been used for the staining of cells and blood films.It is required for the staining of azurophil granules and is an excellent stain for inclusion bodies.Its applications can be seen in hematology for blood and bone marrow specimens, bacteriology, clinical cytology specimens, histological biopsies, and tumor samples.

적합성

Certified for Giemsa stain for blood films and for the Wolbach′s Giemsa method for paraffin sections.

기타 정보

Nuclei will be varying shades of purple. Cytoplasmic staining will be varying shades of blue to light pink. Fine reddish to lilac granules may be present in cytoplasm of some cell types. Basophils will demonstrate dark blue black granules in the cytoplasm. Eosinophils will demonstrate bright orange granules in the cytoplasm. Red blood cells should be pink to orange

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Gloves


시험 성적서(COA)

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