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일반 설명
Sephadex G-75 is widely used for desalting and buffer exchange. This gel filtration resin is created through the crosslinking of dextran with epichlorohydrin. Sephadex varies in its cross-linking level across different types, influencing its swelling capacity and molecular fractionation range.
Sephadex® G-75 is a gel filtration media used in gel filtration chromatography and protein chromatography. Sephadex® G-75 has been used to study cystatin of the mammalian brain, suggest candidates for development of novel oral or other anti-infective agents and to purify procoagulant metalloproteinase from rattlesnake venom.
애플리케이션
Sephadex® G-75 has been used to purify low density lipoproteins (LDL) by gel filtration.
Sephadex® G-75 has been used to separate the products from the pepsin digest by gel filtration chromatography and to desalt low density lipoproteins (LDL) by gel filtration.
특징 및 장점
- Enables rapid desalting, contaminant removal, and buffer exchange in a single step.
- Ideal for buffer exchange and cleanup of large biomolecules with a molecular weight exceeding 80,000.
- Features the highest exclusion limit of 80,000 within the Sephadex G-range.
- Classic gel filtration resin.
법적 정보
Sephadex is a registered trademark of Cytiva
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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시험 성적서(COA)
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이미 열람한 고객
Jose Munoz-Munoz et al.
mBio, 12(4), e0136821-e0136821 (2021-08-04)
The human gut microbiota (HGM) contributes to the physiology and health of its host. The health benefits provided by dietary manipulation of the HGM require knowledge of how glycans, the major nutrients available to this ecosystem, are metabolized. Arabinogalactan proteins
Oxidized low density lipoproteins potentiate vasoconstrictions to various agonists by direct interaction with vascular smooth muscle.
Galle J, et al.
Circulation Research, 66(5), 1287-1293 (1990)
Integrin alpha IIb beta 3 (platelet GPIIb-IIIa) recognizes multiple sites in fibronectin.
Bowditch, RD, et al.
Journal of biological and chemical chronicles, 266(34), 23323-23328 (1991)
Inhibition of cyclic AMP-and cyclic GMP-mediated dilations in isolated arteries by oxidized low density lipoproteins.
Galle J, et al.
Arteriosclerosis and Thrombosis, 12(2), 180-186 (1992)
Effects of LDL on intracellular free calcium and nitric oxide-dependent cGMP formation in porcine endothelial cells
Pohl U, et al.
Atherosclerosis, 117(2), 169-178 (1995)
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