G7770

β-Glucuronidase from Helix pomatia

Type HP-2S, aqueous solution, ≥90,000 units/mL

• 동의어(들): β-D-Glucuronide glucuronosohydrolase
• CAS Number: 9001-45-0
• 효소 위원회 번호: 3.2.1.31 (BRENDA, IUBMB)
• MDL number: MFCD00130629
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 무균: sterile-filtered
• Quality Level: 200
• 유형: Type HP-2S
• 양식: aqueous solution
• 특이 활성도: ≥90,000 units/mL
• secondary activity: ≤7,500 units/mL sulfatase
• 저장 온도: 2-8°C

설명

일반 설명

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

애플리케이션

β-Glucuronidase from Helix pomatia has been used:

• in the treatment of heparinized plasma sample for the hydrolysis of glucuronic acid-conjugated metabolites[1]
• in the pre-treatment of plasma sample for the determination of 1′-hydroxymidazolam (1′-OHMDZ)[2]
• for the treatment of bile samples from rainbow trouts for the hydrolysis of glucuronate and sulfate metabolites[3]
• in treating the cells from the sporulation media for the complete digestion of the cell walls of asci[4]

β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSⁿ) analysis, to study the structures of degradation products of baicalin.[5] It is used as a reporter gene in GUS assays to monitor gene expression.

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

생화학적/생리학적 작용

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

품질

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

단위 정의

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

물리적 형태

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1