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AB5804

Sigma-Aldrich

Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody

serum, Chemicon®

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Synonym(s):
glial fibrillary acidic protein
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, bovine, canine, rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GFAP(2670)

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This Item
MAB3402AB5541MAB360
antibody form

serum

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

ascites fluid

clone

polyclonal

clone

GA5, monoclonal

clone

polyclonal

clone

GA5, monoclonal

species reactivity

human, bovine, canine, rat

species reactivity

chicken, pig, rat, bovine, mouse, rabbit, human

species reactivity

human, mouse, rat, bovine, pig

species reactivity

rat, human, mouse, rabbit, chicken, pig, bovine

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

General description

Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.

Specificity

It is expected that the antibody will react with most mammalian GFAP due to homology. Reactivity with other species has not been confirmed.
Recognizes Glial Fibrillary Acidic Protein (GFAP).

Immunogen

Purified bovine GFAP

Application

Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody is an antibody against Glial Fibrillary Acidic Protein (GFAP) for use in IC, IH, IH(P) with more than 65 product citations.
Immunohistochemistry(paraffin):
Representative images from a previous lot.

Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in Immunocytochemistry.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Quality

Routinely evaluated by immunohistochemistry on brain tissue, astrocytes, and neurons.

Immunohistochemistry(paraffin) Analysis:
GFAP (IHC2078) staining of Human Brain. Tissue pretreated with Citrate Buffer, pH 6.0. Pre-diluted polyclonal antibody, using IHC-Select Detection with HRP-DAB. Glial cells stain strongly (brown).
Optimal Staining with Citrate Buffer Epitope Retrieval: Human Brain

Target description

50 kDa

Linkage

Replaces: AB1540

Physical form

Rabbit serum. Liquid. Contains no preservative.
Unpurified

Storage and Stability

Stable for 1 year at -20°C undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Mouse brain tissue, rat astrocytes, rat neurons.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Toth, G; Zraly, CB; Thomson, TL; Jones, C; Lapetino, S; Muraskas, J; Zhang, J; Dingwall, AK
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PloS one, 4(8), e6617-e6617 (2009-08-13)
DNA methylation is capable of modulating coordinate expression of large numbers of genes across many different pathways, and may therefore warrant investigation for their potential role between genes and disease phenotype. In a rare set of monozygotic twins discordant for
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