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MAB3402C3

Sigma-Aldrich

Anti-GFAP Antibody, Cy3 Conjugate

from mouse, CY3 conjugate

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Synonym(s):
Glial fibrillary acidic protein, GFAP
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

monoclonal

species reactivity

mouse, pig

species reactivity (predicted by homology)

porcine (based on 100% sequence homology), human (based on 100% sequence homology), rat (based on 100% sequence homology), chicken (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GFAP(2670)

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This Item
MAB3402BMAB3402C9205
conjugate

CY3 conjugate

conjugate

biotin conjugate

conjugate

-

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

monoclonal

clone

monoclonal

clone

GA5, monoclonal

clone

G-A-5, monoclonal

species reactivity

mouse, pig

species reactivity

pig, mouse

species reactivity

chicken, pig, rat, bovine, mouse, rabbit, human

species reactivity

human, pig, rat

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable

technique(s)

immunohistochemistry: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

direct immunofluorescence: 1:400 using alcohol-fixed, paraffin-embedded rat brain sections

General description

Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.

Specificity

The antibody reacts with mouse GFAP. In tissue sections this
antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).

Immunogen

Purified glial filament protein (Debus, E., 1983).

Application

Anti-GFAP Antibody, Cy3 Conjugate is an antibody against GFAP for use in IC, IH.
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected GFAP in adult mouse brain tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Immunocytochemistry in differentiated ReNcell VMs.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GFAP in differentiated ReNcell VMs.

Target description

50 kDa Observed (Using unconjugated antibody MAB3402)

Physical form

Protein A purified
Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Differentiated ReNcell VMs

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Ariana Z Turk et al.
The Journal of comparative neurology, 530(2), 574-589 (2021-08-14)
Astrocytes, the star-shaped glial cells, are the most abundant non-neuronal cell population in the central nervous system. They play a key role in modulating activities of neural networks, including those involved in complex motor behaviors. Common marmosets (Callithrix jacchus), the
Kimberley Delaunay et al.
Journal of clinical medicine, 10(13) (2021-07-20)
The aim of this study was to evaluate the potential anti-angiogenic effect of MTRN (meteorin) in the laser-induced CNV rat model and explore its mechanisms of action. MTRN, thrompospondin-1, glial cell markers (GFAP, vimentin), and phalloidin were immuno-stained in non-human
Junjin Lin et al.
Scientific reports, 11(1), 23569-23569 (2021-12-09)
Microvascular compression of the trigeminal root entry zone (TREZ) is the main cause of most primary trigeminal neuralgia (TN), change of glial plasticity was previously studied in the TREZ of TN rat model induced by chronic compression. To better understand
Peng Lu et al.
Investigative ophthalmology & visual science, 63(3), 18-18 (2022-03-17)
Pituitary adenylate cyclase-activating polypeptide (PACAP) has shown potent neuroprotective effects in central nervous system and retina disorders. However, whether PACAP can attenuate retinal neurodegeneration induced by acute ocular hypertension (AOH) and the underlying mechanisms remain unknown. In this study, we
Galina Dvoriantchikova et al.
Scientific reports, 12(1), 17152-17152 (2022-10-14)
Ischemia-reperfusion (IR) injury is implicated in a large array of pathological conditions in the retina. Increasing experimental evidence suggests that programmed necrosis makes a significant contribution to inflammation and retinal damage triggered by IR. Since there are many types of

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