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G4546

Sigma-Aldrich

Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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Synonym(s):
GFAP Antibody for Flow Cytometry - Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit, Gfap Antibody Flow Cytometry, Anti-FLJ45472, Anti-GFAP, Anti-Intermediate filament protein
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

human, rat, mouse

concentration

~1 mg/mL

technique(s)

flow cytometry: 1:200-1:500
immunohistochemistry: 1:100-1:200
western blot: 1:500-1:1,000

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GFAP(2670)
mouse ... Gfap(14580)
rat ... Gfap(24387)

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This Item
G9269G6171C9205
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

CY3 conjugate

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

purified from hybridoma cell culture, purified immunoglobulin

antibody form

purified immunoglobulin

clone

polyclonal

clone

polyclonal

clone

G-A-5, monoclonal

clone

G-A-5, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen ~50 kDa

mol wt

-

mol wt

antigen ~50 kDa

mol wt

-

General description

Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological, and biochemical criteria. Intermediate filaments differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments, and are components of most eukaryotic cells. Glial Fibrillary Acidic Protein (GFAP) (molecular weight of 50 kDa) is found in astrocytes and ependymal cells of the central nervous system. It is found in various other cell types including stellate cells, chondrocytes, osteocytes, keratinocytes, Leydig cells and fibroblasts. GFAP is involved in mitosis, and cell:cell interactions and communications. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system.

Specificity

Rabbit polyclonal anti-Glial Fibrillary Acidic Protein recognizes human, mouse, and rat GFAPs.

Immunogen

synthetic peptide corresponding to an internal C-terminal sequence of human GFAP.

Application

Rabbit polyclonal anti-GFAP antibody is used to tag glial fibrillary acidic protein for detection and quantitation by Western blotting and immunohistochemical (IHC) techniques. It is used as a probe to determine the roles of glial fibrillary acidic protein in cell:cell communications, mitosis and cell structure. Anti-GFAP has proven a valuable tool for use in immunocytochemical localization of GFAP in normal central nervous system tissue, certain tumors and metastases of the glial antigen, as well as for immunofluorescent labeling of cultured mammalian cells.

Physical form

solution in phosphate buffered saline, containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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