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AB9354

Sigma-Aldrich

Anti-Beta III Tubulin Antibody

Chemicon®, from chicken

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Synonym(s):
Anti-CDCBM, Anti-CDCBM1, Anti-CFEOM3, Anti-CFEOM3A, Anti-FEOM3, Anti-TUBB4, Anti-beta-4
eCl@ss:
32160702
NACRES:
NA.41

biological source

chicken

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgY

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TUBB3(10381)
mouse ... Tubb3(22152)
rat ... Tubb3(246118)

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This Item
SAB4200792T5076T7816
Anti-Beta III Tubulin Antibody Chemicon®, from chicken

AB9354

Anti-Beta III Tubulin Antibody

antibody form

affinity purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

ascites fluid

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

clone

polyclonal

clone

ONS.1A6, monoclonal

clone

SDL.3D10, monoclonal

clone

SAP.4G5, monoclonal

UniProt accession no.

Q13509

UniProt accession no.

P04350

UniProt accession no.

Q13509

UniProt accession no.

P07437

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General description

Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division, and intracellular trafficking. There are at least three types of tubulins: alpha, beta, and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents. Class III β-tubulin‎ is a microtubule element expressed exclusively in neurons, and is a popular marker specific for neurons in tissue.

Specificity

Recognizes Beta III Tubulin

Immunogen

Epitope: (Similar to TuJ1 antigen)
Synthetic peptides from human/rat Beta III Tubulin.

Application

Anti-Beta III Tubulin Antibody detects level of Beta III Tubulin & has been published & validated for use in IC, IH & WB.
Immunocytochemistry:
1:1000 - 1:2000 dilution from a previous lot was used in immunocytochemistry using 2% paraformaldehyde fixed cells.

Immunohistochemistry:
1:1000 - 1:2000 dilution from a previous lot was used in immunohistochemistry using 2% paraformaldehyde fixed tissues.

Optimal working dilutions must be determined by the end user
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Neurofilament & Neuron Metabolism

Quality

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot:
1:1000 dilution of this lot detected TUBULIN BETA III on 10 μg of mouse brain lysates.

Target description

55 kDa

Linkage

Replaces: 04-1049

Physical form

Affinity purified polyclonal chicken IgY liquid in PBS, pH 7.2 with 0.02% sodium azide.
Four different antibodies were combined to make this product
ImmunoAffinity Purified

Storage and Stability

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
It is recommended that the antibody be stored in the dark. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.

Analysis Note

Control
Brain tissue, Neuronal culture

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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Anesthetics interfere with the polarization of developing cortical neurons.
Mintz, CD; Smith, SC; Barrett, KM; Benson, DL
Journal of Neurosurgical Anesthesiology null
Loïs S Miraucourt et al.
PloS one, 7(1), e29086-e29086 (2012-01-14)
The developing retinotectal system of the Xenopus laevis tadpole is a model of choice for studying visual experience-dependent circuit maturation in the intact animal. The neurotransmitter gamma-aminobutyric acid (GABA) has been shown to play a critical role in the formation
Kunitoshi Chiba et al.
eLife, 4 (2015-07-22)
Mutations in the human telomerase reverse transcriptase (TERT) promoter are the most frequent non-coding mutations in cancer, but their molecular mechanism in tumorigenesis has not been established. We used genome editing of human pluripotent stem cells with physiological telomerase expression
Bonnie J Berry et al.
ACS chemical neuroscience, 9(7), 1693-1701 (2018-05-11)
Alzheimer's disease (AD) is characterized by slow, progressive neurodegeneration leading to severe neurological impairment, but current drug development efforts are limited by the lack of robust, human-based disease models. Amyloid-β (Aβ) is known to play an integral role in AD
Letizia Ferroni et al.
International journal of molecular sciences, 16(3), 4666-4681 (2015-03-05)
Dental pulp tissue supports the vitality of the tooth, but it is particularly vulnerable to external insults, such as mechanical trauma, chemical irritation or microbial invasion, which can lead to tissue necrosis. In the present work, we present an endodontic

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