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AB9260

Sigma-Aldrich

Anti-Ki-67 Antibody

Chemicon®, from rabbit

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Synonym(s):
Anti-Ki-67
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

species reactivity (predicted by homology)

rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MKI67(4288)
mouse ... Mki67(17345)
rat ... Mki67(291234)

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This Item
P6834FCMAB103APSAB5700770
Anti-Ki-67 Antibody Chemicon®, from rabbit

Sigma-Aldrich

AB9260

Anti-Ki-67 Antibody

antibody form

affinity isolated antibody

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

clone

polyclonal

clone

PP-67, monoclonal

clone

Ki-S5, monoclonal

clone

polyclonal

species reactivity

mouse, human

species reactivity

human

species reactivity

human

species reactivity

rat, mouse, human

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Milli-Mark®

manufacturer/tradename

-

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunoblotting: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800 using microwave-treated human tonsil sections, microarray: suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

technique(s)

immunofluorescence: 1:50-1:100, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:2000

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General description

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Specificity

Recognizes Ki-67

Immunogen

Synthetic peptide from human Ki-67

Application

Detect Ki-67 with Anti-Ki-67 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IHC(P) & WB.
Immunocytochemistry:
Confolcal fluorescent analysis of A431 cells using AB9260 rabbit pAB. Actin filaments were labeled with Alexa Fluor 488 -Phalloidin. Nuclear is stained with DAPI. (Nuclear staining).

Western Blot Analysis:
A previous lot of this antibody was used in western blot.

Optimal working dilutions must be determined by the end user.

Quality

Immunohistochemistry(paraffin):
Representative lot data.
This antibody was used on formalin fixed, paraffin embedded tissue at 1:300 dilution for 10 minutes at room temperature. Staining of formalin-fixed tissues requires boiling of tissues in 10 mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Target description

Predicted: 359 kDa MW may be observed at ~345 or 395 kDa

Physical form

Purified rabbit polyclonal in buffer containing 10 mM PBS, pH 7.4 containing BSA and sodium azide.

Analysis Note

Control
Positive Control: Breast carcinoma, lymph node tissue, normal tonsil.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Rosario Pacheco-Marín et al.
Scientific reports, 6, 23373-23373 (2016-03-30)
Oxygen or nutrient deprivation of early stage tumoral spheroids can be used to reliably mimic the initial growth of primary and metastatic cancer cells. However, cancer cell growth during the initial stages has not been fully explored using a genome-wide
Samar Basu et al.
PloS one, 10(10), e0138443-e0138443 (2015-10-03)
Current evidences suggest that expression of Ki67, cyclooxygenase (COX), aromatase, adipokines, prostaglandins, free radicals, β-catenin and α-SMA might be involved in breast cancer pathogenesis. The main objective of this study was to compare expression/localization of these potential compounds in breast
Ganglioside GD3 is required for neurogenesis and long-term maintenance of neural stem cells in the postnatal mouse brain.
Wang, J; Cheng, A; Wakade, C; Yu, RK
The Journal of Neuroscience null
Yixuan Wang et al.
Nature communications, 7, 10481-10481 (2016-01-23)
Epigenetic regulatory complexes play key roles in the modulation of transcriptional regulation underlying neural stem cell (NSC) proliferation and progeny specification. How specific cofactors guide histone demethylase LSD1/KDM1A complex to regulate distinct NSC-related gene activation and repression in cortical neurogenesis
Kuo-Hui Su et al.
Nature cell biology, 18(5), 527-539 (2016-04-05)
To cope with proteotoxic stress, cells attenuate protein synthesis. However, the precise mechanisms underlying this fundamental adaptation remain poorly defined. Here we report that mTORC1 acts as an immediate cellular sensor of proteotoxic stress. Surprisingly, the multifaceted stress-responsive kinase JNK

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