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P6834

Sigma-Aldrich

Monoclonal Anti-Proliferating Cell Protein Ki-67 antibody produced in mouse

clone PP-67, ascites fluid

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Synonym(s):
Anti-Ki-67
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

PP-67, monoclonal

contains

15 mM sodium azide

species reactivity

human

technique(s)

immunoblotting: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800 using microwave-treated human tonsil sections
microarray: suitable

isotype

IgM

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MKI67(4288)

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This Item
WH0004288M1FCMAB103APSAB5300425
Gene Information

human ... MKI67(4288)

Gene Information

human ... MKI67(4288)

Gene Information

human ... MKI67(4288)

Gene Information

human ... MKI67(4288)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

PP-67, monoclonal

clone

7B8, monoclonal

clone

Ki-S5, monoclonal

clone

8D5, monoclonal

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

ascites fluid

conjugate

unconjugated

conjugate

unconjugated

conjugate

Allophycocyanin conjugate

conjugate

unconjugated

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General description

Marker of proliferation Ki-67 (MKI67) consists of a phosphopeptide-binding forkhead-associated (FHA) domain and a protein phosphatase 1 (PP1)-binding site at the amino- terminal, 16 tandem repeats at the central region and leucine and arginine (LR) pairs at the carboxy- terminal. It is expressed at all active phases of the cell cycle. The gene encoding this protein is localized on human chromosome 10.
Marker of proliferation Ki-67 (MKI67- antigen identified by monoclonal antibody) consists of a phosphopeptide-binding forkhead-associated (FHA) domain and a protein phosphatase 1 (PP1)-binding site at the amino- terminal, 16 tandem repeats at the central region and leucine and arginine (LR) pairs at the carboxy- terminal. It is expressed at all active phases (G1, S, G2, and mitosis) of the cell cycle. The gene encoding this protein is localized on human chromosome 10.

Specificity

Reacts with the Ki-67 nuclear antigen (345 and 395 kDa, and several lower m.w. proteins) in immunoblotting. The product shows an immunohistochemical staining pattern similar to that obtained with other anti-Ki-67/MIB-1 reagents. It labels proliferating cells in frozen human tissue and in formalin-fixed, paraffin-embedded tissue. A microwave heating retrieval step is required for successful labeling of routinely formalin-fixed, paraffin-embedded human tissue.

Immunogen

HeLa cell nuclear preparation.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry.
Monoclonal Anti-Proliferating Cell Protein Ki-67 antibody produced in mouse has also been used for immunohistochemistry.

Biochem/physiol Actions

Marker of proliferation Ki-67 (MKI67), which is a part of the mitotic chromosome periphery, acts as a biological surfactant to maintain individual mitotic chromosomes dispersed in the cytoplasm after nuclear envelope disassembly. It is a prognostic marker in breast and prostate cancer. MKI67 is also a potential cell proliferation marker.
Marker of proliferation Ki-67 (MKI67- antigen identified by monoclonal antibody), which is a part of the mitotic chromosome periphery, acts as a biological surfactant to maintain individual mitotic chromosomes dispersed in the cytoplasm after nuclear envelope disassembly. It is a prognostic marker in breast and prostate cancer. MKI67 is also a potential cell proliferation marker. Ki-67 is considered as poor prognostic factors of adenoid cystic carcinoma.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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VEGF and Ki-67 overexpression in predicting poor overall survival in adenoid cystic carcinoma.
Park S, et al.
Cancer Research and Treatment : Official Journal of Korean Cancer Association, 48(2), 518-518 (2016)
The Ki-67 protein: from the known and the unknown.
Scholzen T and Gerdes J
Journal of Cellular Physiology. Supplement (2000)
Xuebao Xiang et al.
Oncology letters, 15(3), 3049-3055 (2018-02-13)
Recent advances have highlighted the important roles of long non-coding RNAs (lncRNAs) in a number of biological processes, including oncogenesis. However, the function of lncRNA cartilage injury-related (lncRNA-CIR) in bladder cancer progression remains elusive. A novel function for lncRNA-CIR in
Ki67 labelling index: an independent predictor of progression in prostate cancer treated by radical prostatectomy.
Bubendorf L
The Journal of Pathology (1996)
Pathological studies and detection of different bovine papilloma virus types in buffalo cutaneous warts.
Jangir B L, et al.
Turkish Journal of Veterinary and Animal Sciences, 41(2), 306-311 (2017)

Articles

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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