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G7017

Sigma-Aldrich

β-Glucuronidase from Helix pomatia

Type HP-2, aqueous solution, ≥100,000 units/mL

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Synonym(s):
β-D-Glucuronide glucuronosohydrolase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

biological source

Helix pomatia

Quality Level

type

Type HP-2

form

aqueous solution

specific activity

≥100,000 units/mL

secondary activity

≤7,500 units/mL sulfatase

shipped in

wet ice

storage temp.

2-8°C

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G0762G8885G0876
vibrant-m

G7017

β-Glucuronidase from Helix pomatia

vibrant-m

G0762

β-Glucuronidase from Helix pomatia

vibrant-m

G8885

β-Glucuronidase from Helix pomatia

vibrant-m

G0876

β-Glucuronidase from Helix pomatia

biological source

Helix pomatia

biological source

Helix pomatia

biological source

-

biological source

-

form

aqueous solution

form

aqueous solution

form

aqueous solution

form

aqueous solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

300

General description

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the digestive juices of the Roman snail. β-glucuronidases derived from mollusks often contain sulfatase activity. Both activities are useful in the hydrolysis of metabolites for toxicology and biomarker analysis. For a broader look at our β-Glucuronidase product line and their uses see our page about Drug Metabolite Analysis

Application

  • Sample preparation for LC/MS through digestion of glucuronide conjugated compounds for biomarker and toxicology research.
  • Removal of glucuronide conjugates such as for the preparation of Baicalein.­
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

Endogenous beta-glucuronidase is a lysosomal enzyme involved in digestion of glucuronide-modified catabolites.
Microbial beta-glucuronidase found in microbiota can cause re-absorption of metabolites by hydrolysis of glucuronide conjugates of xenobiotic and endobiotic metabolites.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Features and Benefits

  • pH optima 4.0 - 5.0.
  • Sulfatase pH optima 6.2
  • Low Sulfatase activity (<7,500 units/mL).
  • Ammonium Sulfate suspension.
  • Mollusk-derived beta-glucuronidase.
  • Useful for acidic hydrolysis of glucuronides in low pH samples.

Quality

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Linkage

Similar to G0876, but produced by Sigma.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Physical form

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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W Dennis Ulrey et al.
Journal of AOAC International, 96(4), 917-924 (2013-09-05)
A multilaboratory study of AOAC Official Method 2011.23 was performed to satisfy requirements for Final Action status through the AOAC expert review panel process. The study included nine collaborating laboratories from the United States, Canada, Brazil, and The Netherlands. Five
Mladen Boban et al.
Journal of cardiovascular pharmacology, 47(5), 695-701 (2006-06-16)
By using red wine (RW), dealcoholized red wine (DARW), polyphenols-stripped red wine (PSRW), ethanol-water solution (ET), and water (W), the role of wine polyphenols, ethanol, and urate on vascular function was examined in humans (n = 9 per beverage) and
L Bláhová et al.
Journal of dairy science, 99(9), 6973-6982 (2016-07-11)
Isoflavones are natural phytoestrogens with antioxidant and endocrine-disrupting potencies. Monitoring of their levels is important to ensure the high quality and safety of food, milk, and dairy products. The efficiency and accuracy of phytoestrogen analyses in complex matrices such as
Jack R T Darby et al.
The Journal of physiology, 597(20), 5063-5077 (2019-09-05)
Substrate restriction during critical developmental windows of gestation programmes offspring for a predisposition towards cardiovascular disease in adult life. This study aimed to determine the effect of maternal resveratrol (RSV) treatment in an animal model in which chronic fetal catheterisation
Madalina Neacsu et al.
Molecular nutrition & food research, 61(3) (2016-07-01)
Phytophenols present in cereals are metabolised to compounds that could be partly responsible for the reduced risk of chronic diseases and all-cause mortality associated with fibre-rich diets. The bioavailability, form and in vivo concentrations of these metabolites require to be

Articles

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Protocols

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver. This procedure applies to all b-Glucuronidase products that are derived from Helix Pomatia and Bovine Liver. It is the responsibility of trained Analytical Services laboratory personnel to follow this procedure as written.

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