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GE17-0575-01

Chelating Sepharose™ Fast Flow

Cytiva 17-0575-01, pack of 50 mL

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About This Item

UNSPSC 코드:
41106500
NACRES:
NA.56

ligand

iminodiacetic acid

포장

pack of 50 mL

제조업체/상표

Cytiva 17-0575-01

Matrix

6% cross-linked agarose

입자 크기

45-165 μm

평균 직경

90 μm

cleaning in place

2-14

작동 범위

3-13

적합성

suitable for bioprocess medium

관련 카테고리

일반 설명

Chelating Sepharose™ Fast Flow is a BioProcess IMAC medium, designed for purifying native and recombinant proteins and peptides that have an affinity for metal ions.

Chelating Sepharose™ Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.

Chelating Sepharose™ Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.

Chelating Sepharose™ Fast Flow is available in a range of different bulk pack sizes for easy scale-up and process development. As member of the BioProcess media range, Chelating Sepharose™ Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support

특징 및 장점

  • Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
  • Convenient purification of native and recombinant proteins
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • BioProcess medium supported for industrial applications and well-established in approved processes

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

Sepharose is a trademark of Cytiva

픽토그램

Flame
GHS02

신호어

Warning

유해 및 위험 성명서

H226

Storage Class Code

3 - Flammable liquids

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시험 성적서(COA)

Lot/Batch Number

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이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

Immobilized Metal Chelate Affinity Chromatography (IMAC)

How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.

Purification or Removal of Proteins and Peptides

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

프로토콜

Separation with Cytiva Products

Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

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