I5271

IL-1β mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 동의어(들): IL-1β, Lymphocyte activating factor
• MDL number: MFCD00164815
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• product name: Interleukin-1β from mouse, IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• 생물학적 소스: mouse
• Quality Level: 200
• 재조합: expressed in E. coli
• 분석: ≥97% (SDS-PAGE)
• 형태: lyophilized powder
• 효능: ≤0.010 ng/mL ED₅₀
• 품질: endotoxin tested
• 분자량: 17.5 kDa (containing 153 amino acid residues)
• 포장: pkg of 5X5 μg; pkg of 5 μg
• 기술: cell culture | mammalian: suitable
• 불순물: ≤0.1ng/mg
• UniProt 수납 번호: P10749
• 저장 온도: −20°C
• 유전자 정보: mouse ... Il1b(16176)

설명

일반 설명

Interleukin-1β (IL-1β) cytokine is generated by monocytes, dendritic cells, macrophages and keratinocytes. Pro-IL-1β, an IL-1β precursor undergoes proteolytic cleavage to produce its active form of 17 kDa.

애플리케이션

Interleukin-1β from mouse has been used:

• as a proinflammatory agent, to induce inflammatory signaling cascades mediated cellular damage
• to study its effect on fetuin A-induced inflammatory response in podocyte
• to check its effect on the expression of mitogen-activated protein kinase 4 using 3T3-L1 adipocytes

생화학적/생리학적 작용

Interleukin-1β (IL-1β) is a potent inflammatory cytokine, and mediates inflammation through proinflammatory molecules: cyclooxygenase-2 (COX2) and nitric oxide. IL-1β release is stimulated by injury and increased levels are observed in response to severe pain. It has its main function in T-cell activation, antigen recognition and T-helper cell 17 cell development. IL-1β is also responsible for the formation of IL-17 and IL-22 by nature killer T (NKT) cells and NK cells. It is known to induce inflammation in psoriasis and rheumatoid arthritis. IL-1β controls intercellular adhesion molecule-1 expression. It is significantly associated with bone damage and vascular injury.

물리적 형태

Lyophilized from 0.2 μm filtered solution in 10mM Sodium Citrate, pH 4.0

분석 메모

The biological activity is measured by the dose-dependent stimulation of murine D10G4.1 cells.

기타 정보

The two closely related agents, Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) bind to the same cell surface receptor, elicit nearly identical biological responses and yet share 25% homology in their amino acid sequence.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable