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Merck
모든 사진(2)

주요 문서

L2380

Sigma-Aldrich

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

lyophilized powder

동의어(들):

Bandeirea simplicifolia agglutinin, BS-I

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About This Item

UNSPSC 코드:
12352202
NACRES:
NA.32

양식

lyophilized powder

Quality Level

효능

<20 μg per mL agglutination activity (using human blood group B erythrocytes)

구성

Protein, ≥90% biuret

불순물

salt, free

저장 온도

2-8°C

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생화학적/생리학적 작용

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

기타 정보

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

제조 메모

Purified by affinity chromatography using the method of Hayes, C. E., and Goldstein, I. J., J. Biol. Chem., 249, 1904 (1974).

분석 메모

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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