L2879

Lysozyme chloride form from chicken egg white

Grade VI, ≥35,000 units/mg protein (E1%/282)

• 동의어(들): Peptidoglycan iV-acetylmuramoyl hydrolase, Mucopeptide N-acetylmuramoylhydrolase, Muramidase
• CAS Number: 9066-59-5
• 효소 위원회 번호: 3.2.1.17 (BRENDA, IUBMB)
• EC Number: 232-954-0
• MDL number: MFCD00131557
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: chicken egg white
• Quality Level: 200
• 유형: Grade VI
• 양식: lyophilized powder
• 특이 활성도: ≥35,000 units/mg protein (E1%/282)
• 분자량: ~14.3 kDa
• 구성: Protein, ≥80%
• 기술: cell based assay: suitable
• 적합성: suitable for cell lysis
• 응용 분야: cell analysis

설명

일반 설명

Lysozyme, also known as muramidase, is a bacteriolytic enzyme found in phagocytic cells like leukocytes. The name "lysozyme" is derived from its ability to dissolve or hydrolyze the β-1-4-glycosidic bond between N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) in the walls of certain bacteria.[1] This protein is present in animals, plants, bacteria, and viruses. It can also be found in granules of neutrophils, macrophages, as well as in serum, saliva, milk, honey, and hen egg white. There are different types of lysozymes, including the c-type (chicken type), the g-type (goose-type), and the i-type (invertebrates).[2]

애플리케이션

Lysozyme chloride form from chicken egg white has been used:

• as a component in the protein mixture to study the effects of yeast cells on membrane fouling caused by a protein mixture in dead-end filtration[3]
• in dip system to study its bacteriostatic or bactericidal effects on Salmonella-contaminated broiler parts[4]
• in viscometric assay for determining lysozyme activity[5]    

생화학적/생리학적 작용

Lysozyme serves as an indicator of macrophage-mediated host response, correlates with white cell death, and exhibits a high turnover rate. Elevated levels of serum lysozyme have been observed in various chronic inflammatory conditions, inflammatory bowel diseases, hematological disorders, and renal disorders.[1] The c-type lysozyme from hen egg white is commonly used as a model for studying protein structure and function. Muramidase primarily exhibits bactericidal activity against Gram-positive bacteria.[2]

단위 정의

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

물리적 형태

Lyophilized powder containing sodium chloride and sodium acetate

제조 메모

3× Crystallized

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)