4403-M

Lysozyme, Chicken Egg White

Native, chicken egg white lysozyme. Inhibits the biological activity of lipopolysaccharides from periodontopathic bacteria.

• Synonym(s): Lysozyme, Chicken Egg White, Muramidase, N-Acetylmuramyl Hydrolase
• CAS Number: 12650-88-3
• Enzyme Commission number: 3.2.1.17 (BRENDA, IUBMB)
• MDL number: MFCD00131557
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: chicken egg white
• form: lyophilized solid
• specific activity: ≥20,000 Shugar units/mg dry wt
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• technique(s): cell based assay: suitable
• color: off-white
• solubility: cold water: 5 mg/mL; aqueous buffer: soluble
• suitability: suitable for cell lysis
• application(s): cell analysis
• storage temp.: −20°C

Description

General description

Native, chicken egg white lysozyme. Hydrolyzes β1,4 linkages between N-acetylmuramic acid (2-acetamido-2-deoxy-3-O-[1carboxymethyl]-glucopyranose) and N-acetyl-glucosamine residues in chitin. Also hydrolyzes β1,4 linkages between N-acetylmuramic acid and N-acetyl-glucosamine residues in peptidoglycans. Inhibits the biological activity of lipopolysaccharides from periodontopathic bacteria.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One Shugar unit is defined as the amount of enzyme that will cause a decrease in absorbance of 0.001 at 450 nm per min due to lysis of Micrococcus luteus at 25°C, pH 6.2.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 year at -20°C.

Other Notes

Ito, H.O., et al. 1997. J. Periodontal Res. 32, 295.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable