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Key Documents

M3770

Sigma-Aldrich

Micrococcus lysodeikticus ATCC No. 4698

suitable for substrate for the assay of lysozyme, lyophilized cells

Synonym(s):

Micrococcus luetus

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized cells

Quality Level

200

suitability

suitable for substrate for the assay of lysozyme

storage temp.

−20°C

Application

Micrococcus lysodeikticus ATCC No. 4698 has been used in a study to assess lysozyme separation by hollow-fibre ultrafiltration. It has also been used in a study to investigate the encapsulation of protein drugs in biodegradable microparticles.
Lysozyme lysates harvested from cultures of Micrococcus lysodeikticus were attached to sepharose and used for affinity chromatography to isolate various bacteriolytic enzymes.

Biochem/physiol Actions

Micrococcus luetus is a Gram-positive bacteria that is identified by the release of yellow water-insoluble pigments. This species requires succinic acid for its growth and is found to be susceptible to β-lytic metalloendopeptidase lyses by Lysobacter enzymogenes. Its membrane includes enzymes that participate in the prenylation reactions by utilizing prenyl pyrophosphates as donors. M. luteus is known to be used for cloning the cis-prenyl transferase gene.

Quality

Contains polynucleotide phosphorylase.

Unit Definition

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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Cecilia C Sánchez et al.
BMC genomics, 12, 626-626 (2011-12-23)
Fish under intensive culture conditions are exposed to a variety of acute and chronic stressors, including high rearing densities, sub-optimal water quality, and severe thermal fluctuations. Such stressors are inherent in aquaculture production and can induce physiological responses with adverse
John D Steemson et al.
PloS one, 9(1), e86050-e86050 (2014-01-28)
The OB-fold is a small, versatile single-domain protein binding module that occurs in all forms of life, where it binds protein, carbohydrate, nucleic acid and small-molecule ligands. We have exploited this natural plasticity to engineer a new class of non-immunoglobulin
Tania Jauslin et al.
mBio, 12(1) (2021-02-18)
Ingestion and killing of bacteria by phagocytic cells protect the human body against infections. While many mechanisms have been proposed to account for bacterial killing in phagosomes, their relative importance, redundancy, and specificity remain unclear. In this study, we used
Thais Heloisa Vaz Farias et al.
Fish & shellfish immunology, 101, 186-191 (2020-04-05)
Aeromonas hydrophila is responsible for outbreaks of a severe infectious disease in fish farms around the world and is one of the major causes of economic losses to the neotropical fish farmers. This study assessed the induction of immune responses
Ghosh et al.
Biochemical engineering journal, 6(1), 19-24 (2000-07-26)
This paper discusses the purification of lysozyme from chicken egg white using hollow-fibre ultrafiltration (30kDa MWCO, polysulphone membrane). Lysozyme is preferentially transmitted through the membrane while the membrane largely retains other egg white proteins. Improvement in system hydrodynamics resulted in
View All Related Papers

Protocols

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

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