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Merck
모든 사진(1)

문서

M7905

Sigma-Aldrich

Monoclonal Anti-Myosin Light Chain Kinase antibody produced in mouse

clone K36, ascites fluid

동의어(들):

Anti-KRP, Anti-MLCK, Anti-MLCK1, Anti-MLCK108, Anti-MLCK210, Anti-MMIHS, Anti-MMIHS1, Anti-MSTP083, Anti-MYLK1, Anti-smMLCK

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.44

생물학적 소스

mouse

결합

unconjugated

항체 형태

ascites fluid

항체 생산 유형

primary antibodies

클론

K36, monoclonal

포함

15 mM sodium azide

종 반응성

avian, mammals

기술

immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:10,000

동형

IgG2b

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... MYLK(4638)

일반 설명

Monoclonal Anti-Myosin Light Chain Kinase (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Myosin light chain kinase (MLCK) is a calcium/calmodulin-dependent enzyme (approx.160 kD). It is expressed in skeletal, cardiac, smooth and mammalian non-muscle cells. MLCK is encoded by the MYLK1 gene. This gene encodes telokin, non-muscle and the smooth muscle isoforms of MLCK. MLCK gene is mapped to the human chromosome location 3q21.1.

특이성

In immunoblotting, the antibody recognizes the myosin light chain kinase of smooth muscle from various sources including chicken gizzard, turkey gizzard, and pig stomach as well as the myosin light chain kinase of non-muscle cells such as cultured fibroblasts.

면역원

chicken gizzard myosin light chain kinase.

애플리케이션

Monoclonal anti-myosin light chain kinase antibody has been used in:
  • immunohistochemistry
  • immunoblot (diluted 1:10,000) analysis
  • flow cytometry

생화학적/생리학적 작용

Myosin Light Chain Kinase (MLCK) has a pivotal role in phosphorylating myosin regulatory light chains and hence facilitates its interaction with actin filaments for smooth muscle contraction. Monoclonal anti-myosin light chain kinase antibody can be used for studying the function of the enzyme and its interactions with other cell components. It can also be used in microarray and immunoprecipitation. Mouse anti-myosin light chain kinase antibody reacts specifically with myosin light chain kinase present in smooth muscle of chicken gizzard, turkey gizzard, and pig stomach. This product also shows reactivity for myosin light chain kinase of non-muscle cells like cultured fibroblasts.
The catalytic subunit of cardiac and skeletal muscle myosin light chain kinase is in the range of 80-95 kDa. Proteolysis of the myosin light chain kinase from chicken gizzard produces a 64 kDa fragment that neither binds to Ca2+/calmodulin nor exhibits catalytic activity and a 61 kDa peptide that is active in the absence of Ca2+/calmodulin.

물리적 형태

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

저장 및 안정성

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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