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Merck
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Key Documents

M8662

Sigma-Aldrich

Mineral oil

PCR Reagent

동의어(들):

Mineral oil for PCR

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
12181504
NACRES:
NA.52

Grade

PCR Reagent

형태

liquid

포장

vial of 1.5 mL (Total volume 7.5 mL (5 vials))

기술

PCR: suitable

색상

colorless

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

외래 활성

DNase, RNase, protease, none detected

저장 온도

room temp

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Mineral oil is used as a reaction mix overlay in polymerase chain reactions (PCR) to prevent reaction solutions from evaporating during the thermal cycling in PCR instruments without heated lids.

애플리케이션

Mineral oil has been used:
  • for routine PCR amplifications
  • to improve the heat conductivity between the device and the cycler during reverse transcription-polymerase chain reaction (RT–PCR) amplification
  • to prevent evaporation during single-cell MATQ-sequencing performed on Bravo automated liquid handling platform

특징 및 장점

  • Provided in a convenient 5 × 1.5 mL (1 vial) pack size
  • Tested for the absence of DNase, RNase, and protease.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

No data available

Flash Point (°C)

No data available

개인 보호 장비

Eyeshields, Gloves


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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