추천 제품
사용
sufficient for 100 colorimetric tests
검출 방법
colorimetric
관련 질환(들)
cancer; orthopedic diseases; aging/geriatric diseases
저장 온도
−20°C
유전자 정보
human ... PFKL(5211) , PFKM(5213) , PFKP(5214)
mouse ... PFKL(18641) , PFKM(18642) , PFKP(56421)
rat ... PFKL(25741) , PFKM(65152) , PFKP(60416)
일반 설명
Phosphofructokinase (PFK) catalyzes the third step of glycolysis, the conversion of fructose-6-phosphate to fructose-1,6-diphosphate, the rate limiting step of glycolysis. PFK activity is highly regulated by multiple cofactors in addition to post-translational modifications and can be used to measure glycolytic flux in tissues. Deficiencies in PFK activity can result in the glycogen storage disease, glycogenosis type VII (Tarui′s disease), which is a glycogen storage disease.
애플리케이션
Phosphofructokinase (PFK) Activity Colorimetric Assay Kit has been used to measure the activity of phosphofructokinase.
적합성
Suitable for the measurement of phosphofructokinase activity, analysis of glucose metabolism and cell signaling and screening anti-cancer drugs in animal tissues (liver, brain, heart, muscles etc.) and cell culture (adherent or suspension cells).
원리
The Phosphofructokinase Colorimetric Assay kit provides a simple and direct procedure for measuring PFK activity in a variety of samples. PFK activity is determined by a coupled enzyme assay, in which fructose-6-phosphate and ATP is converted to fructose-1,6-diphosphate and ADP by PFK. The ADP is converted by the enzyme mix to AMP and NADH. The resulting NADH reduces a colorless probe resulting in a colorimetric (450 nm) product proportional to the PFK activity present. One unit of PFK is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 7.4 at 37 °C.
신호어
Danger
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
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시험 성적서(COA)
Lot/Batch Number
Neng Tang et al.
Biochemical and biophysical research communications, 526(1), 1-7 (2020-03-21)
The mechanisms underlying the initiation and proliferation of liver regeneration (LR) has been extensively studied using the partial hepatectomy (PHx) model, while little is known about the termination of LR. PP2Acα (protein phosphatase 2 A catalytic subunit α isoform) is
Manipulation of a glycolytic regulator alters growth and carbon partitioning in the marine diatom Thalassiosira pseudonana.
Abbriano R, et al.
Algal research, 32, 250-258 (2018)
Emilio Bueno et al.
Nature microbiology, 3(12), 1346-1353 (2018-10-03)
To survive and proliferate in the absence of oxygen, many enteric pathogens can undergo anaerobic respiration within the host by using nitrate (NO3-) as an electron acceptor1,2. In these bacteria, NO3- is typically reduced by a nitrate reductase to nitrite
Kai Zhou et al.
Cell death & disease, 9(10), 988-988 (2018-09-27)
Plastic phenotype convention between glioma stem cells (GSCs) and non-stem tumor cells (NSTCs) significantly fuels glioblastoma heterogeneity that causes therapeutic failure. Recent progressions indicate that glucose metabolic reprogramming could drive cell fates. However, the metabolic pattern of GSCs and NSTCs
D S Matassa et al.
Cell death and differentiation, 23(9), 1542-1554 (2016-05-21)
Tumour cells have long been considered defective in mitochondrial respiration and mostly dependent on glycolytic metabolism. However, this assumption is currently challenged by several lines of evidence in a growing number of tumours. Ovarian cancer (OC) is one of the
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