MAK144

Fluorometric Intracellular Ros Kit

sufficient for 200 fluorometric tests (orange)

• 동의어(들): Fluorometric ROS Assay Kit
• EC Number: 200-664-3
• UNSPSC 코드: 12161503
• NACRES: NA.25

속성

• 사용: sufficient for 200 fluorometric tests (orange)
• 응용 분야: cosmetics; food and beverages
• 검출 방법: fluorometric
• 관련 질환(들): cancer; neurological disorders
• 저장 온도: −20°C

설명

일반 설명

Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell.[1] At physiological levels, ROS contribute to cell signaling and host defense.[2] Increased ROS generation above the biological system′s detoxification capacity results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA.[1][2] ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.[3]

애플리케이션

Fluorometric intracellular reactive oxygen species (ROS) Kit has been used to evaluate the mitochondrial damage by mechanical ventilation (MV).[4] It is also used to measure the production of intracellular ROS.[5]

적합성

This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader, flow cytometer, or a fluorescent microscope with TRITC filter.

원리

The Fluorometric Intracellular ROS Assay Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (λ_ex = 540/λ_em = 570 nm) proportional to the amount of ROS present. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable