추천 제품
포장
pkg of 1 kit
Quality Level
제조업체/상표
Distributed for Phanos Technologies
저장 조건
protect from light
기술
flow cytometry: suitable
형광
λex 490 nm; λem 502 nm (PKH67 dye)
응용 분야
cell analysis
detection
검출 방법
fluorometric
배송 상태
ambient
저장 온도
room temp
일반 설명
This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
애플리케이션
PKH67 Green Fluorescent Cell Linker Midi Kit has been used for:
- labeling exosomes
- extracellular vesicles (EVs)l
- normal and sickle red blood cells (RBCs)
- Raji cells
결합
For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.
법적 정보
CellVue is a registered trademark of Phanos Technologies
키트 구성품 전용
제품 번호
설명
- PKH67 Dye 2 x 0.1
- Diluent C 6 x 10
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Storage Class Code
3 - Flammable liquids
Flash Point (°F)
57.2 °F - closed cup
Flash Point (°C)
14 °C - closed cup
이미 열람한 고객
Dongxin Tang et al.
Clinical and translational medicine, 10(4), e139-e139 (2020-09-09)
Cancer stem cells (CSCs) are important factors contributing to tumorigenesis. We examined whether CSCs isolated from colorectal cancer (CRC) cells possess metastatic properties that can be transferred to non-CSCs via the delivery of miR-200c enclosed in extracellular vesicles (EVs). The
Notochordal-cell derived extracellular vesicles exert regenerative effects on canine and human nucleus pulposus cells
Bach F, et al.
Testing, 8(51), 88845-88845 (2017)
ADCC Assay Protocol
Fang L and He F
Journal of Virology (2013)
Chuang Gao et al.
Aging, 11(24), 12147-12164 (2019-12-17)
Exosomes are small (30-150 nm diameter) lipid bilayer-enclosed vesicles found in all bodily fluids. We investigated whether exosomes play a role in chronic subdural hematoma (CSDH). Exosomes were identified and characterized using transmission electron microscopy and NanoSight particle tracking. The
Modulation of Sickle Red Blood Cell Adhesion and its Associated Changes in Biomarkers by Sulfated Nonanticoagulant Heparin Derivative
Alshaiban A, et al.
Clinical and Applied Thrombosis/Hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 22, 230-238 (2016)
문서
Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.
PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.