N1127

2-Nitrophenyl β-D-galactopyranoside

≥98% (enzymatic)

• 동의어(들): ONPG , o-Nitrophenyl β-D-galactopyranoside, ONPG
• 실험식(Hill 표기법): C₁₂H₁₅NO₈
• CAS Number: 369-07-3
• Molecular Weight: 301.25
• Beilstein: 92207
• EC Number: 206-716-1
• MDL number: MFCD00063255
• UNSPSC 코드: 12352200
• PubChem Substance ID: 24897461
• NACRES: NA.52

속성

• Grade: for molecular biology
• Quality Level: 200
• 분석: ≥98% (enzymatic)
• 형태: powder
• 저장 온도: −20°C
• SMILES string: OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1
• InChI key: KUWPCJHYPSUOFW-YBXAARCKSA-N

설명

일반 설명

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.

애플리케이션

2-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase.

생화학적/생리학적 작용

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

원리

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

재구성

A stock solution can be prepared in molecular biology grade water at a concentration of 3 mg/ml. Alternatively, a stock solution of approximately 20.5 mg/ml is prepared in 100 mM sodium phosphate buffer (pH 7.3). Gentle warming may be required to completely dissolve the product.

기질

Chromogenic substrate for β-galactosidase

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)