N7653
p-Nitrophenyl Phosphate Liquid Substrate System
alkaline phosphatase substrate, liquid
동의어(들):
4-Nitrophenyl phosphate disodium salt solution, pNPP
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모든 사진(3)
About This Item
Linear Formula:
O2NC6H4OP(O)(ONa)2
CAS Number:
Molecular Weight:
263.05
MDL number:
UNSPSC 코드:
12352204
PubChem Substance ID:
NACRES:
NA.83
추천 제품
제품명
p-Nitrophenyl Phosphate Liquid Substrate System, liquid
양식
liquid
Quality Level
배송 상태
wet ice
저장 온도
−20°C
SMILES string
[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI
1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
InChI key
VIYFPAMJCJLZKD-UHFFFAOYSA-L
일반 설명
The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.
애플리케이션
p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
생화학적/생리학적 작용
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.
주의사항
Stable at least one year when stored below 0 °C.
물리적 형태
Ready-to-use.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
표적 기관
Kidney,Liver,Blood
Storage Class Code
8B - Non-combustible corrosive hazardous materials
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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문서
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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